Stringent doxycycline-dependent control of gene activities using an episomal one-vector system

Conditional expression systems are of pivotal importance for the dissection of complex biological phenomena. Here, we describe a novel EBV-derived episomally replicating plasmid (pRTS-1) that carries all the elements for conditional expression of a gene of interest via Tet regulation. The vector is...

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Detalles Bibliográficos
Autores principales: Bornkamm, Georg W., Berens, Christian, Kuklik-Roos, Conny, Bechet, Jean-Marie, Laux, Gerhard, Bachl, Jürgen, Korndoerfer, Martin, Schlee, Martin, Hölzel, Michael, Malamoussi, Anastassia, Chapman, Rob D., Nimmerjahn, Falk, Mautner, Josef, Hillen, Wolfgang, Bujard, Hermann, Feuillard, Jean
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201338/
https://www.ncbi.nlm.nih.gov/pubmed/16147984
http://dx.doi.org/10.1093/nar/gni137

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