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Thermal difference spectra: a specific signature for nucleic acid structures
We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201377/ https://www.ncbi.nlm.nih.gov/pubmed/16157860 http://dx.doi.org/10.1093/nar/gni134 |
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author | Mergny, Jean-Louis Li, Jing Lacroix, Laurent Amrane, Samir Chaires, Jonathan B. |
author_facet | Mergny, Jean-Louis Li, Jing Lacroix, Laurent Amrane, Samir Chaires, Jonathan B. |
author_sort | Mergny, Jean-Louis |
collection | PubMed |
description | We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures above and below its melting temperature (T(m)). The difference between these two spectra is the TDS. The TDS has a specific shape that is unique for each type of nucleic acid structure, a conclusion that is based on a comparison of >900 spectra from 200 different sequences. The shape of the TDS reflects the subtleties of base stacking interactions that occur uniquely within each type of nucleic acid structure. TDS provides a simple, inexpensive and rapid method to obtain structural insight into nucleic acid structures, which is applicable to both DNA and RNA from short oligomers to polynucleotides. TDS complements circular dichroism as a tool for the structural characterization of nucleic acids in solution. |
format | Text |
id | pubmed-1201377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-12013772005-09-15 Thermal difference spectra: a specific signature for nucleic acid structures Mergny, Jean-Louis Li, Jing Lacroix, Laurent Amrane, Samir Chaires, Jonathan B. Nucleic Acids Res Methods Online We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures above and below its melting temperature (T(m)). The difference between these two spectra is the TDS. The TDS has a specific shape that is unique for each type of nucleic acid structure, a conclusion that is based on a comparison of >900 spectra from 200 different sequences. The shape of the TDS reflects the subtleties of base stacking interactions that occur uniquely within each type of nucleic acid structure. TDS provides a simple, inexpensive and rapid method to obtain structural insight into nucleic acid structures, which is applicable to both DNA and RNA from short oligomers to polynucleotides. TDS complements circular dichroism as a tool for the structural characterization of nucleic acids in solution. Oxford University Press 2005 2005-09-12 /pmc/articles/PMC1201377/ /pubmed/16157860 http://dx.doi.org/10.1093/nar/gni134 Text en © The Author 2005. Published by Oxford University Press. All rights reserved |
spellingShingle | Methods Online Mergny, Jean-Louis Li, Jing Lacroix, Laurent Amrane, Samir Chaires, Jonathan B. Thermal difference spectra: a specific signature for nucleic acid structures |
title | Thermal difference spectra: a specific signature for nucleic acid structures |
title_full | Thermal difference spectra: a specific signature for nucleic acid structures |
title_fullStr | Thermal difference spectra: a specific signature for nucleic acid structures |
title_full_unstemmed | Thermal difference spectra: a specific signature for nucleic acid structures |
title_short | Thermal difference spectra: a specific signature for nucleic acid structures |
title_sort | thermal difference spectra: a specific signature for nucleic acid structures |
topic | Methods Online |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201377/ https://www.ncbi.nlm.nih.gov/pubmed/16157860 http://dx.doi.org/10.1093/nar/gni134 |
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