Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C

BACKGROUND: Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS: A met...

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Detalles Bibliográficos
Autores principales: Ailenberg, Menachem, Goldenberg, Neil M, Silverman, Mel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1208860/
https://www.ncbi.nlm.nih.gov/pubmed/16137330
http://dx.doi.org/10.1186/1472-6750-5-23

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