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Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics

BACKGROUND: The purpose of our study was to investigate the immunohistochemical expression of TGF-β1 and p27 in pancreatic adenocarcinomas and to compare the findings with the clinicopathological features and survival. We also aimed to evaluate the expression of TGF-β1 and p27 in the context of othe...

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Autores principales: Culhaci, Nil, Sagol, Ozgul, Karademir, Sedat, Astarcioglu, Huseyin, Astarcioglu, Ibrahim, Soyturk, Mujde, Oztop, Ilhan, Obuz, Funda
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1208869/
https://www.ncbi.nlm.nih.gov/pubmed/16086840
http://dx.doi.org/10.1186/1471-2407-5-98
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author Culhaci, Nil
Sagol, Ozgul
Karademir, Sedat
Astarcioglu, Huseyin
Astarcioglu, Ibrahim
Soyturk, Mujde
Oztop, Ilhan
Obuz, Funda
author_facet Culhaci, Nil
Sagol, Ozgul
Karademir, Sedat
Astarcioglu, Huseyin
Astarcioglu, Ibrahim
Soyturk, Mujde
Oztop, Ilhan
Obuz, Funda
author_sort Culhaci, Nil
collection PubMed
description BACKGROUND: The purpose of our study was to investigate the immunohistochemical expression of TGF-β1 and p27 in pancreatic adenocarcinomas and to compare the findings with the clinicopathological features and survival. We also aimed to evaluate the expression of TGF-β1 and p27 in the context of other cell cycle and proliferation markers such as cyclin D1 and Ki-67. METHODS: We examined TGF-β1 and p27 expression immunohistochemically in 63 cases of invasive ductal adenocarcinoma of the pancreas. Standard streptavidin-biotin immunperoxidase method was used for immunostaining and the stained slides were examined microscopically using semiquantitative criteria. RESULTS: TGF-β1 stained the cytoplasms of the tumor cells in 43 cases [68.3%]. There was a statistically significant difference among TGF-β1 staining scores in terms of clinicopathologic factors such as blood vessel invasion, stage and distant metastasis [p < 0.05]. Of the 63 tumors evaluated 23 [36.5%] were positive for p27 within the nucleus. An inverse correlation was found between p27 immunoreactivity and grade [p < 0.05]. But no significant correlation was found between p27 and other parameters. Among the patients with survival data 27 patients had RO resections and these cases were considered in survival analysis. In the univariate analysis, neither TGF-β1 nor p27 expression was related with patient survival. CONCLUSION: Our findings suggest that in pancreatic carcinoma, TGF-β1 expression is related to tumor growth and metastasis. But it is not associated with cell cycle proteins. p27 expression is reduced in pancreatic adenocarcinomas and decreased protein levels of p27 may play a role in the differentiation of pancreatic cancer.
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spelling pubmed-12088692005-09-15 Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics Culhaci, Nil Sagol, Ozgul Karademir, Sedat Astarcioglu, Huseyin Astarcioglu, Ibrahim Soyturk, Mujde Oztop, Ilhan Obuz, Funda BMC Cancer Research Article BACKGROUND: The purpose of our study was to investigate the immunohistochemical expression of TGF-β1 and p27 in pancreatic adenocarcinomas and to compare the findings with the clinicopathological features and survival. We also aimed to evaluate the expression of TGF-β1 and p27 in the context of other cell cycle and proliferation markers such as cyclin D1 and Ki-67. METHODS: We examined TGF-β1 and p27 expression immunohistochemically in 63 cases of invasive ductal adenocarcinoma of the pancreas. Standard streptavidin-biotin immunperoxidase method was used for immunostaining and the stained slides were examined microscopically using semiquantitative criteria. RESULTS: TGF-β1 stained the cytoplasms of the tumor cells in 43 cases [68.3%]. There was a statistically significant difference among TGF-β1 staining scores in terms of clinicopathologic factors such as blood vessel invasion, stage and distant metastasis [p < 0.05]. Of the 63 tumors evaluated 23 [36.5%] were positive for p27 within the nucleus. An inverse correlation was found between p27 immunoreactivity and grade [p < 0.05]. But no significant correlation was found between p27 and other parameters. Among the patients with survival data 27 patients had RO resections and these cases were considered in survival analysis. In the univariate analysis, neither TGF-β1 nor p27 expression was related with patient survival. CONCLUSION: Our findings suggest that in pancreatic carcinoma, TGF-β1 expression is related to tumor growth and metastasis. But it is not associated with cell cycle proteins. p27 expression is reduced in pancreatic adenocarcinomas and decreased protein levels of p27 may play a role in the differentiation of pancreatic cancer. BioMed Central 2005-08-08 /pmc/articles/PMC1208869/ /pubmed/16086840 http://dx.doi.org/10.1186/1471-2407-5-98 Text en Copyright © 2005 Culhaci et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Culhaci, Nil
Sagol, Ozgul
Karademir, Sedat
Astarcioglu, Huseyin
Astarcioglu, Ibrahim
Soyturk, Mujde
Oztop, Ilhan
Obuz, Funda
Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
title Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
title_full Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
title_fullStr Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
title_full_unstemmed Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
title_short Expression of transforming growth factor-beta-1 and p27(Kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
title_sort expression of transforming growth factor-beta-1 and p27(kip1 )in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1208869/
https://www.ncbi.nlm.nih.gov/pubmed/16086840
http://dx.doi.org/10.1186/1471-2407-5-98
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