Constraining ribosomal RNA conformational space

Despite the potential for many possible secondary-structure conformations, the native sequence of ribosomal RNA (rRNA) is able to find the correct and universally conserved core fold. This study reports a computational analysis investigating two mechanisms that appear to constrain rRNA secondary-str...

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Detalles Bibliográficos
Autores principales: Favaretto, Paola, Bhutkar, Arjun, Smith, Temple F.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Computational Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1214544/
https://www.ncbi.nlm.nih.gov/pubmed/16155182
http://dx.doi.org/10.1093/nar/gki805
Descripción
Sumario:Despite the potential for many possible secondary-structure conformations, the native sequence of ribosomal RNA (rRNA) is able to find the correct and universally conserved core fold. This study reports a computational analysis investigating two mechanisms that appear to constrain rRNA secondary-structure conformational space: ribosomal proteins and rRNA sequence composition. The analysis was carried out by using rRNA–ribosomal protein interaction data for the Escherichia coli 16S rRNA and free energy minimization software for secondary-structure prediction. The results indicate that selection pressures on rRNA sequence composition and ribosomal protein–rRNA interaction play a key role in constraining the rRNA secondary structure to a single stable form.

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