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Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons
BACKGROUND: Messenger RNA (mRNA) comprises three major parts: a 5'-UTR (UnTranslated Region), a coding region, and a 3'-UTR. The 3'-UTR contains signal sequences involved in polyadenylation, degradation and localization/stabilization processes. Some sequences in the 3'-UTR are in...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1215523/ https://www.ncbi.nlm.nih.gov/pubmed/16120223 http://dx.doi.org/10.1186/1742-4682-2-33 |
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author | Aranda-Abreu, Gonzalo E Hernández, Ma Elena Soto, Abraham Manzo, Jorge |
author_facet | Aranda-Abreu, Gonzalo E Hernández, Ma Elena Soto, Abraham Manzo, Jorge |
author_sort | Aranda-Abreu, Gonzalo E |
collection | PubMed |
description | BACKGROUND: Messenger RNA (mRNA) comprises three major parts: a 5'-UTR (UnTranslated Region), a coding region, and a 3'-UTR. The 3'-UTR contains signal sequences involved in polyadenylation, degradation and localization/stabilization processes. Some sequences in the 3'-UTR are involved in the localization of mRNAs in (e.g.) neurons, epithelial cells, oocytes and early embryos, but such localization has been most thoroughly studied in neurons. Neuronal polarity is maintained by the microtubules (MTs) found along both dendrites and axon and is partially influenced by sub-cellular mRNA localization. A widely studied mRNA is that for Tau protein, which is located in the axon hillock and growth cone; its localization depends on the well-characterized cis-acting signal (U-rich region) in the 3'-UTR. METHODS: We compared the cis-acting signal of Tau with mRNAs in the axonal regions of neurons using the ClustalW program for alignment of sequences and the Mfold program for analysis of secondary structures. RESULTS: We found that at least 3 out of 12 mRNA analyzed (GRP75, cofilin and synuclein) have a sequence similar to the cis-acting signal of Tau in the 3'-UTR. This could indicate that these messengers are localized specifically in the axon. The Mfold program showed that these mRNAs have a similar "bubble" structure in the putative sequence signal. CONCLUSION: Hence, we suggest that a U-rich sequence in the 3'-UTR region of the mRNA could act as a signal for its localization in the axon in neuronal cells. Sequences homologous to the DTE sequence of BC1 mRNA could direct the messenger to the dendrites. Messengers with homologues of both types of sequence, e.g. β-actin, might be located in both dendrites and axon. |
format | Text |
id | pubmed-1215523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-12155232005-09-17 Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons Aranda-Abreu, Gonzalo E Hernández, Ma Elena Soto, Abraham Manzo, Jorge Theor Biol Med Model Research BACKGROUND: Messenger RNA (mRNA) comprises three major parts: a 5'-UTR (UnTranslated Region), a coding region, and a 3'-UTR. The 3'-UTR contains signal sequences involved in polyadenylation, degradation and localization/stabilization processes. Some sequences in the 3'-UTR are involved in the localization of mRNAs in (e.g.) neurons, epithelial cells, oocytes and early embryos, but such localization has been most thoroughly studied in neurons. Neuronal polarity is maintained by the microtubules (MTs) found along both dendrites and axon and is partially influenced by sub-cellular mRNA localization. A widely studied mRNA is that for Tau protein, which is located in the axon hillock and growth cone; its localization depends on the well-characterized cis-acting signal (U-rich region) in the 3'-UTR. METHODS: We compared the cis-acting signal of Tau with mRNAs in the axonal regions of neurons using the ClustalW program for alignment of sequences and the Mfold program for analysis of secondary structures. RESULTS: We found that at least 3 out of 12 mRNA analyzed (GRP75, cofilin and synuclein) have a sequence similar to the cis-acting signal of Tau in the 3'-UTR. This could indicate that these messengers are localized specifically in the axon. The Mfold program showed that these mRNAs have a similar "bubble" structure in the putative sequence signal. CONCLUSION: Hence, we suggest that a U-rich sequence in the 3'-UTR region of the mRNA could act as a signal for its localization in the axon in neuronal cells. Sequences homologous to the DTE sequence of BC1 mRNA could direct the messenger to the dendrites. Messengers with homologues of both types of sequence, e.g. β-actin, might be located in both dendrites and axon. BioMed Central 2005-08-24 /pmc/articles/PMC1215523/ /pubmed/16120223 http://dx.doi.org/10.1186/1742-4682-2-33 Text en Copyright © 2005 Aranda-Abreu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Aranda-Abreu, Gonzalo E Hernández, Ma Elena Soto, Abraham Manzo, Jorge Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons |
title | Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons |
title_full | Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons |
title_fullStr | Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons |
title_full_unstemmed | Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons |
title_short | Possible Cis-acting signal that could be involved in the localization of different mRNAs in neuronal axons |
title_sort | possible cis-acting signal that could be involved in the localization of different mrnas in neuronal axons |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1215523/ https://www.ncbi.nlm.nih.gov/pubmed/16120223 http://dx.doi.org/10.1186/1742-4682-2-33 |
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