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Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein
BACKGROUND: To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV). RESULTS: HBV particles were produced in culture medium of HepG2 cells transfected with the mammalian expression vector containing the linear HBV genome...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1215529/ https://www.ncbi.nlm.nih.gov/pubmed/16135253 http://dx.doi.org/10.1186/1743-422X-2-76 |
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author | Lwa, Siew Hui Chen, Wei Ning |
author_facet | Lwa, Siew Hui Chen, Wei Ning |
author_sort | Lwa, Siew Hui |
collection | PubMed |
description | BACKGROUND: To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV). RESULTS: HBV particles were produced in culture medium of HepG2 cells transfected with the mammalian expression vector containing the linear HBV genome, as assessed by commercially available ELISA assay. A cDNA library was made from these cells exposed to HBV. From yeast two hybrid screening with HBX as bait, human guanine nucleotide binding protein β subunit 5L (GNβ5) was isolated from the cDNA library constructed in this study as a new HBX-interacting protein. The HBX-GNβ5 interaction was further supported by mammalian two hybrid assay. CONCLUSION: The use of a cDNA library constructed from HBV-transfected HepG2 cells has resulted in the isolation of new cellular proteins interacting with HBX. |
format | Text |
id | pubmed-1215529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-12155292005-09-17 Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein Lwa, Siew Hui Chen, Wei Ning Virol J Short Report BACKGROUND: To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV). RESULTS: HBV particles were produced in culture medium of HepG2 cells transfected with the mammalian expression vector containing the linear HBV genome, as assessed by commercially available ELISA assay. A cDNA library was made from these cells exposed to HBV. From yeast two hybrid screening with HBX as bait, human guanine nucleotide binding protein β subunit 5L (GNβ5) was isolated from the cDNA library constructed in this study as a new HBX-interacting protein. The HBX-GNβ5 interaction was further supported by mammalian two hybrid assay. CONCLUSION: The use of a cDNA library constructed from HBV-transfected HepG2 cells has resulted in the isolation of new cellular proteins interacting with HBX. BioMed Central 2005-08-31 /pmc/articles/PMC1215529/ /pubmed/16135253 http://dx.doi.org/10.1186/1743-422X-2-76 Text en Copyright © 2005 Lwa and Chen; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Lwa, Siew Hui Chen, Wei Ning Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein |
title | Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein |
title_full | Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein |
title_fullStr | Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein |
title_full_unstemmed | Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein |
title_short | Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein |
title_sort | hepatitis b virus x protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1215529/ https://www.ncbi.nlm.nih.gov/pubmed/16135253 http://dx.doi.org/10.1186/1743-422X-2-76 |
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