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GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells
Gfi-1B (growth factor independence-1B) gene is an erythroid-specific transcription factor, whose expression plays an essential role in erythropoiesis. Our laboratory has previously defined the human Gfi-1B promoter region and shown that GATA-1 mediates erythroid-specific Gfi-1B transcription. By fur...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1226314/ https://www.ncbi.nlm.nih.gov/pubmed/16177182 http://dx.doi.org/10.1093/nar/gki838 |
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author | Huang, Duen-Yi Kuo, Yuan-Yeh Chang, Zee-Fen |
author_facet | Huang, Duen-Yi Kuo, Yuan-Yeh Chang, Zee-Fen |
author_sort | Huang, Duen-Yi |
collection | PubMed |
description | Gfi-1B (growth factor independence-1B) gene is an erythroid-specific transcription factor, whose expression plays an essential role in erythropoiesis. Our laboratory has previously defined the human Gfi-1B promoter region and shown that GATA-1 mediates erythroid-specific Gfi-1B transcription. By further investigating the regulation of the Gfi-1B promoter, here we report that (i) Gfi-1B transcription is negatively regulated by its own gene product, (ii) GATA-1, instead of Gfi-1B, binds directly to the Gfi-1-like sites in the Gfi-1B promoter and (iii) Gfi-1B suppresses GATA-1-mediated stimulation of Gfi-1B promoter through their protein interaction. These results not only demonstrate that interaction of GATA-1 and Gfi-1B participates in a feedback regulatory pathway in controlling the expression of the Gfi-1B gene, but also provide the first evidence that Gfi-1B can exert its repression function by acting on GATA-1-mediated transcription without direct binding to the Gfi-1 site of the target genes. Based on these data, we propose that this negative auto-regulatory feedback loop is important in restricting the expression level of Gfi-1B, thus optimizing its function in erythroid cells. |
format | Text |
id | pubmed-1226314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-12263142005-09-27 GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells Huang, Duen-Yi Kuo, Yuan-Yeh Chang, Zee-Fen Nucleic Acids Res Article Gfi-1B (growth factor independence-1B) gene is an erythroid-specific transcription factor, whose expression plays an essential role in erythropoiesis. Our laboratory has previously defined the human Gfi-1B promoter region and shown that GATA-1 mediates erythroid-specific Gfi-1B transcription. By further investigating the regulation of the Gfi-1B promoter, here we report that (i) Gfi-1B transcription is negatively regulated by its own gene product, (ii) GATA-1, instead of Gfi-1B, binds directly to the Gfi-1-like sites in the Gfi-1B promoter and (iii) Gfi-1B suppresses GATA-1-mediated stimulation of Gfi-1B promoter through their protein interaction. These results not only demonstrate that interaction of GATA-1 and Gfi-1B participates in a feedback regulatory pathway in controlling the expression of the Gfi-1B gene, but also provide the first evidence that Gfi-1B can exert its repression function by acting on GATA-1-mediated transcription without direct binding to the Gfi-1 site of the target genes. Based on these data, we propose that this negative auto-regulatory feedback loop is important in restricting the expression level of Gfi-1B, thus optimizing its function in erythroid cells. Oxford University Press 2005 2005-09-21 /pmc/articles/PMC1226314/ /pubmed/16177182 http://dx.doi.org/10.1093/nar/gki838 Text en © The Author 2005. Published by Oxford University Press. All rights reserved |
spellingShingle | Article Huang, Duen-Yi Kuo, Yuan-Yeh Chang, Zee-Fen GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells |
title | GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells |
title_full | GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells |
title_fullStr | GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells |
title_full_unstemmed | GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells |
title_short | GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells |
title_sort | gata-1 mediates auto-regulation of gfi-1b transcription in k562 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1226314/ https://www.ncbi.nlm.nih.gov/pubmed/16177182 http://dx.doi.org/10.1093/nar/gki838 |
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