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Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients

BACKGROUND: While numerous studies have characterized primary ovarian tumors, little information is available regarding expression patterns of metastatic sites of this cancer. To define sets of genes that distinguish primary and metastatic ovarian tumors, we used cDNA microarrays to characterize glo...

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Autores principales: Schaner, Marci E, Davidson, Ben, Skrede, Martina, Reich, Reuven, Flørenes, Vivi Ann, Risberg, Björn, Berner, Aasmund, Goldberg, Iris, Givant-Horwitz, Vered, Tropè, Claes G, Kristensen, Gunnar B, Nesland, Jahn M, Børresen-Dale, Anne-Lise
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1236614/
https://www.ncbi.nlm.nih.gov/pubmed/16042759
http://dx.doi.org/10.1186/1476-4598-4-26
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author Schaner, Marci E
Davidson, Ben
Skrede, Martina
Reich, Reuven
Flørenes, Vivi Ann
Risberg, Björn
Berner, Aasmund
Goldberg, Iris
Givant-Horwitz, Vered
Tropè, Claes G
Kristensen, Gunnar B
Nesland, Jahn M
Børresen-Dale, Anne-Lise
author_facet Schaner, Marci E
Davidson, Ben
Skrede, Martina
Reich, Reuven
Flørenes, Vivi Ann
Risberg, Björn
Berner, Aasmund
Goldberg, Iris
Givant-Horwitz, Vered
Tropè, Claes G
Kristensen, Gunnar B
Nesland, Jahn M
Børresen-Dale, Anne-Lise
author_sort Schaner, Marci E
collection PubMed
description BACKGROUND: While numerous studies have characterized primary ovarian tumors, little information is available regarding expression patterns of metastatic sites of this cancer. To define sets of genes that distinguish primary and metastatic ovarian tumors, we used cDNA microarrays to characterize global gene expression patterns in 38 effusions (28 peritoneal, 10 pleural) and 8 corresponding primary ovarian tumors, and searched for associations between expression patterns and clinical parameters. RESULTS: We observed multidimensional variation in expression patterns among the cancers. Coordinate variation in expression of genes from two chromosomal regions, 8q and 19q, was seen in subsets of the cancers indicating possible amplifications in these regions. A set of 112 unique genes of known function was differentially expressed between primary tumors and effusions using supervised analysis. Relatively few differences were seen between effusions isolated from the pleural and peritoneal cavities or between effusions from patients diagnosed with stage III and stage IV cancers. A set of 84 unique genes was identified that distinguished high from lower grade ovarian cancers. The results were corroborated using immunocytochemistry, mRNA in situ hybridization, and immunoblotting. CONCLUSION: The extensive variation in expression patterns observed underscores the molecular heterogeneity of ovarian cancer, but suggests a similar molecular profile for ovarian carcinoma cells in serosal cavities.
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spelling pubmed-12366142005-09-28 Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients Schaner, Marci E Davidson, Ben Skrede, Martina Reich, Reuven Flørenes, Vivi Ann Risberg, Björn Berner, Aasmund Goldberg, Iris Givant-Horwitz, Vered Tropè, Claes G Kristensen, Gunnar B Nesland, Jahn M Børresen-Dale, Anne-Lise Mol Cancer Research BACKGROUND: While numerous studies have characterized primary ovarian tumors, little information is available regarding expression patterns of metastatic sites of this cancer. To define sets of genes that distinguish primary and metastatic ovarian tumors, we used cDNA microarrays to characterize global gene expression patterns in 38 effusions (28 peritoneal, 10 pleural) and 8 corresponding primary ovarian tumors, and searched for associations between expression patterns and clinical parameters. RESULTS: We observed multidimensional variation in expression patterns among the cancers. Coordinate variation in expression of genes from two chromosomal regions, 8q and 19q, was seen in subsets of the cancers indicating possible amplifications in these regions. A set of 112 unique genes of known function was differentially expressed between primary tumors and effusions using supervised analysis. Relatively few differences were seen between effusions isolated from the pleural and peritoneal cavities or between effusions from patients diagnosed with stage III and stage IV cancers. A set of 84 unique genes was identified that distinguished high from lower grade ovarian cancers. The results were corroborated using immunocytochemistry, mRNA in situ hybridization, and immunoblotting. CONCLUSION: The extensive variation in expression patterns observed underscores the molecular heterogeneity of ovarian cancer, but suggests a similar molecular profile for ovarian carcinoma cells in serosal cavities. BioMed Central 2005-07-21 /pmc/articles/PMC1236614/ /pubmed/16042759 http://dx.doi.org/10.1186/1476-4598-4-26 Text en Copyright © 2005 Schaner et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Schaner, Marci E
Davidson, Ben
Skrede, Martina
Reich, Reuven
Flørenes, Vivi Ann
Risberg, Björn
Berner, Aasmund
Goldberg, Iris
Givant-Horwitz, Vered
Tropè, Claes G
Kristensen, Gunnar B
Nesland, Jahn M
Børresen-Dale, Anne-Lise
Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients
title Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients
title_full Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients
title_fullStr Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients
title_full_unstemmed Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients
title_short Variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients
title_sort variation in gene expression patterns in effusions and primary tumors from serous ovarian cancer patients
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1236614/
https://www.ncbi.nlm.nih.gov/pubmed/16042759
http://dx.doi.org/10.1186/1476-4598-4-26
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