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Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals.
We developed a DNA microarray to evaluate the estrogen activity of natural estrogens and industrial chemicals. Using MCF-7 cells, we conducted a comprehensive analysis of estrogen-responsive genes among approximately 20,000 human genes. On the basis of reproducible and reliable responses of the gene...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1241992/ https://www.ncbi.nlm.nih.gov/pubmed/15159206 |
| _version_ | 1782125559122231296 |
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| author | Terasaka, Shunichi Aita, Yukie Inoue, Akio Hayashi, Shinichi Nishigaki, Michiko Aoyagi, Kazuhiko Sasaki, Hiroki Wada-Kiyama, Yuko Sakuma, Yasuo Akaba, Shuichi Tanaka, Junko Sone, Hideko Yonemoto, Junzo Tanji, Masao Kiyama, Ryoiti |
| author_facet | Terasaka, Shunichi Aita, Yukie Inoue, Akio Hayashi, Shinichi Nishigaki, Michiko Aoyagi, Kazuhiko Sasaki, Hiroki Wada-Kiyama, Yuko Sakuma, Yasuo Akaba, Shuichi Tanaka, Junko Sone, Hideko Yonemoto, Junzo Tanji, Masao Kiyama, Ryoiti |
| author_sort | Terasaka, Shunichi |
| collection | PubMed |
| description | We developed a DNA microarray to evaluate the estrogen activity of natural estrogens and industrial chemicals. Using MCF-7 cells, we conducted a comprehensive analysis of estrogen-responsive genes among approximately 20,000 human genes. On the basis of reproducible and reliable responses of the genes to estrogen, we selected 172 genes to be used for developing a customized DNA microarray. Using this DNA microarray, we examined estrogen activity among natural estrogens (17beta-estradiol, estriol, estrone, genistein), industrial chemicals (diethylstilbestrol, bisphenol A, nonylphenol, methoxychlor), and dioxin. We obtained results identical to those for other bioassays that are used for detecting estrogen activity. On the basis of statistical correlations analysis, these bioassays have shown more sensitivity for dioxin and methoxychlor. |
| format | Text |
| id | pubmed-1241992 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2004 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-12419922005-11-08 Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. Terasaka, Shunichi Aita, Yukie Inoue, Akio Hayashi, Shinichi Nishigaki, Michiko Aoyagi, Kazuhiko Sasaki, Hiroki Wada-Kiyama, Yuko Sakuma, Yasuo Akaba, Shuichi Tanaka, Junko Sone, Hideko Yonemoto, Junzo Tanji, Masao Kiyama, Ryoiti Environ Health Perspect Research Article We developed a DNA microarray to evaluate the estrogen activity of natural estrogens and industrial chemicals. Using MCF-7 cells, we conducted a comprehensive analysis of estrogen-responsive genes among approximately 20,000 human genes. On the basis of reproducible and reliable responses of the genes to estrogen, we selected 172 genes to be used for developing a customized DNA microarray. Using this DNA microarray, we examined estrogen activity among natural estrogens (17beta-estradiol, estriol, estrone, genistein), industrial chemicals (diethylstilbestrol, bisphenol A, nonylphenol, methoxychlor), and dioxin. We obtained results identical to those for other bioassays that are used for detecting estrogen activity. On the basis of statistical correlations analysis, these bioassays have shown more sensitivity for dioxin and methoxychlor. 2004-05 /pmc/articles/PMC1241992/ /pubmed/15159206 Text en |
| spellingShingle | Research Article Terasaka, Shunichi Aita, Yukie Inoue, Akio Hayashi, Shinichi Nishigaki, Michiko Aoyagi, Kazuhiko Sasaki, Hiroki Wada-Kiyama, Yuko Sakuma, Yasuo Akaba, Shuichi Tanaka, Junko Sone, Hideko Yonemoto, Junzo Tanji, Masao Kiyama, Ryoiti Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. |
| title | Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. |
| title_full | Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. |
| title_fullStr | Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. |
| title_full_unstemmed | Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. |
| title_short | Using a customized DNA microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. |
| title_sort | using a customized dna microarray for expression profiling of the estrogen-responsive genes to evaluate estrogen activity among natural estrogens and industrial chemicals. |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1241992/ https://www.ncbi.nlm.nih.gov/pubmed/15159206 |
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