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Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis
Estrogenic activity in environmental samples could be mediated through a wide variety of compounds and by various mechanisms. High-affinity compounds for estrogen receptors (ERs), such as natural or synthetic estrogens, as well as low-affinity compounds such as alkylphenols, phthalates, and polychlo...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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National Institue of Environmental Health Sciences
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253752/ https://www.ncbi.nlm.nih.gov/pubmed/15743715 http://dx.doi.org/10.1289/ehp.7522 |
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author | Pillon, Arnaud Boussioux, Anne-Marie Escande, Aurélie Aït-Aïssa, Sélim Gomez, Elena Fenet, Hélène Ruff, Marc Moras, Dino Vignon, Françoise Duchesne, Marie-Josèphe Casellas, Claude Nicolas, Jean-Claude Balaguer, Patrick |
author_facet | Pillon, Arnaud Boussioux, Anne-Marie Escande, Aurélie Aït-Aïssa, Sélim Gomez, Elena Fenet, Hélène Ruff, Marc Moras, Dino Vignon, Françoise Duchesne, Marie-Josèphe Casellas, Claude Nicolas, Jean-Claude Balaguer, Patrick |
author_sort | Pillon, Arnaud |
collection | PubMed |
description | Estrogenic activity in environmental samples could be mediated through a wide variety of compounds and by various mechanisms. High-affinity compounds for estrogen receptors (ERs), such as natural or synthetic estrogens, as well as low-affinity compounds such as alkylphenols, phthalates, and polychlorinated biphenyls are present in water and sediment samples. Furthermore, compounds such as polycyclic aromatic hydrocarbons, which do not bind ERs, modulate estrogen activity by means of the aryl hydrocarbon receptor (AhR). In order to characterize compounds that mediate estrogenic activity in river water and sediment samples, we developed a tool based on the ER-αligand-binding domain, which permitted us to estimate contaminating estrogenic compound affinities. We designed a simple transactivation assay in which compounds of high affinity were captured by limited amounts of recombinant ER-αand whose capture led to a selective inhibition of transactivation. This approach allowed us to bring to light that water samples contain estrogenic compounds that display a high affinity for ERs but are present at low concentrations. In sediment samples, on the contrary, we showed that estrogenic compounds possess a low affinity and are present at high concentration. Finally, we used immobilized recombinant ER-αto separate ligands for ER and AhR that are present in river sediments. Immobilized ER-α, which does not retain dioxin-like compounds, enabled us to isolate and concentrate ER ligands to facilitate their further analysis. |
format | Text |
id | pubmed-1253752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | National Institue of Environmental Health Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-12537522005-11-08 Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis Pillon, Arnaud Boussioux, Anne-Marie Escande, Aurélie Aït-Aïssa, Sélim Gomez, Elena Fenet, Hélène Ruff, Marc Moras, Dino Vignon, Françoise Duchesne, Marie-Josèphe Casellas, Claude Nicolas, Jean-Claude Balaguer, Patrick Environ Health Perspect Research Estrogenic activity in environmental samples could be mediated through a wide variety of compounds and by various mechanisms. High-affinity compounds for estrogen receptors (ERs), such as natural or synthetic estrogens, as well as low-affinity compounds such as alkylphenols, phthalates, and polychlorinated biphenyls are present in water and sediment samples. Furthermore, compounds such as polycyclic aromatic hydrocarbons, which do not bind ERs, modulate estrogen activity by means of the aryl hydrocarbon receptor (AhR). In order to characterize compounds that mediate estrogenic activity in river water and sediment samples, we developed a tool based on the ER-αligand-binding domain, which permitted us to estimate contaminating estrogenic compound affinities. We designed a simple transactivation assay in which compounds of high affinity were captured by limited amounts of recombinant ER-αand whose capture led to a selective inhibition of transactivation. This approach allowed us to bring to light that water samples contain estrogenic compounds that display a high affinity for ERs but are present at low concentrations. In sediment samples, on the contrary, we showed that estrogenic compounds possess a low affinity and are present at high concentration. Finally, we used immobilized recombinant ER-αto separate ligands for ER and AhR that are present in river sediments. Immobilized ER-α, which does not retain dioxin-like compounds, enabled us to isolate and concentrate ER ligands to facilitate their further analysis. National Institue of Environmental Health Sciences 2005-03 2004-12-09 /pmc/articles/PMC1253752/ /pubmed/15743715 http://dx.doi.org/10.1289/ehp.7522 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, ?Reproduced with permission from Environmental Health Perspectives?); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright. |
spellingShingle | Research Pillon, Arnaud Boussioux, Anne-Marie Escande, Aurélie Aït-Aïssa, Sélim Gomez, Elena Fenet, Hélène Ruff, Marc Moras, Dino Vignon, Françoise Duchesne, Marie-Josèphe Casellas, Claude Nicolas, Jean-Claude Balaguer, Patrick Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis |
title | Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis |
title_full | Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis |
title_fullStr | Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis |
title_full_unstemmed | Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis |
title_short | Binding of Estrogenic Compounds to Recombinant Estrogen Receptor-α: Application to Environmental Analysis |
title_sort | binding of estrogenic compounds to recombinant estrogen receptor-α: application to environmental analysis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253752/ https://www.ncbi.nlm.nih.gov/pubmed/15743715 http://dx.doi.org/10.1289/ehp.7522 |
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