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Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes

We describe the construction and application of elements for random insertion of promoter containing DNA into the genome of Bacillus subtilis. The outward-facing promoter of these integrative elements termed InsTet(G+) is inducible by tetracycline so that conditional mutants are generated. We constr...

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Detalles Bibliográficos
Autores principales:	Bertram, Ralph, Köstner, Martin, Müller, Judith, Ramos, José Vazquez, Hillen, Wolfgang
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2005
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1253839/ https://www.ncbi.nlm.nih.gov/pubmed/16221969 http://dx.doi.org/10.1093/nar/gni154

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