Cargando…

Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells

BACKGROUND: The greater susceptibility of children to renal injury in post-diarrheal hemolytic-uremic syndrome (HUS) may be related, at least in part, to heightened renal cell sensitivity to the cytotoxic effect of Shiga toxin (Stx), the putative mediator of kidney damage in HUS. We hypothesized tha...

Descripción completa

Detalles Bibliográficos
Autores principales: Hughes, Alisa K, Schmid, Douglas I, Kohan, Donald E
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126269/
https://www.ncbi.nlm.nih.gov/pubmed/12181081
http://dx.doi.org/10.1186/1471-2369-3-6
_version_ 1782120331917393920
author Hughes, Alisa K
Schmid, Douglas I
Kohan, Donald E
author_facet Hughes, Alisa K
Schmid, Douglas I
Kohan, Donald E
author_sort Hughes, Alisa K
collection PubMed
description BACKGROUND: The greater susceptibility of children to renal injury in post-diarrheal hemolytic-uremic syndrome (HUS) may be related, at least in part, to heightened renal cell sensitivity to the cytotoxic effect of Shiga toxin (Stx), the putative mediator of kidney damage in HUS. We hypothesized that sexual maturation, which coincides with a falling incidence of HUS, may induce a relatively Stx-resistant state in the renal cells. METHODS: Cultured human glomerular endothelial (HGEN), human glomerular visceral epithelial (HGEC) and human proximal tubule (HPT) cells were exposed to Stx-1 after pre-incubation with progesterone, β-estradiol or testosterone followed by determination of cytotoxicity. RESULTS: Under basal conditions, Stx-1 potently and dose-dependently killed HPT and HGEC, but had relatively little effect on HGEN. Pre-incubation for 1, 2 or 7 days with physiologic or pharmacologic concentrations of progesterone, β-estradiol or testosterone had no effect on Stx-1 cytotoxicity dose-response on any cell type. In addition, no steroid altered Gb3 expression (Stx receptor) by any cell type at any time point. CONCLUSION: These data do not support the notion that hormonal changes associated with puberty induce an Stx-resistant state within kidney cells.
format Text
id pubmed-126269
institution National Center for Biotechnology Information
language English
publishDate 2002
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-1262692002-09-20 Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells Hughes, Alisa K Schmid, Douglas I Kohan, Donald E BMC Nephrol Research Article BACKGROUND: The greater susceptibility of children to renal injury in post-diarrheal hemolytic-uremic syndrome (HUS) may be related, at least in part, to heightened renal cell sensitivity to the cytotoxic effect of Shiga toxin (Stx), the putative mediator of kidney damage in HUS. We hypothesized that sexual maturation, which coincides with a falling incidence of HUS, may induce a relatively Stx-resistant state in the renal cells. METHODS: Cultured human glomerular endothelial (HGEN), human glomerular visceral epithelial (HGEC) and human proximal tubule (HPT) cells were exposed to Stx-1 after pre-incubation with progesterone, β-estradiol or testosterone followed by determination of cytotoxicity. RESULTS: Under basal conditions, Stx-1 potently and dose-dependently killed HPT and HGEC, but had relatively little effect on HGEN. Pre-incubation for 1, 2 or 7 days with physiologic or pharmacologic concentrations of progesterone, β-estradiol or testosterone had no effect on Stx-1 cytotoxicity dose-response on any cell type. In addition, no steroid altered Gb3 expression (Stx receptor) by any cell type at any time point. CONCLUSION: These data do not support the notion that hormonal changes associated with puberty induce an Stx-resistant state within kidney cells. BioMed Central 2002-08-14 /pmc/articles/PMC126269/ /pubmed/12181081 http://dx.doi.org/10.1186/1471-2369-3-6 Text en Copyright © 2002 Hughes et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Hughes, Alisa K
Schmid, Douglas I
Kohan, Donald E
Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
title Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
title_full Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
title_fullStr Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
title_full_unstemmed Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
title_short Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
title_sort sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126269/
https://www.ncbi.nlm.nih.gov/pubmed/12181081
http://dx.doi.org/10.1186/1471-2369-3-6
work_keys_str_mv AT hughesalisak sexsteroidsdonotaffectshigatoxincytotoxicityonhumanrenaltubularorglomerularcells
AT schmiddouglasi sexsteroidsdonotaffectshigatoxincytotoxicityonhumanrenaltubularorglomerularcells
AT kohandonalde sexsteroidsdonotaffectshigatoxincytotoxicityonhumanrenaltubularorglomerularcells