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Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells

BACKGROUND: Native as well as recombinant bacterial cell surface layer (S-layer) protein of Geobacillus (G.) stearothermophilus ATCC 12980 assembles to supramolecular structures with an oblique symmetry. Upon expression in E. coli, S-layer self assembly products are formed in the cytosol. We tested...

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Detalles Bibliográficos
Autores principales: Blecha, Andreas, Zarschler, Kristof, Sjollema, Klaas A, Veenhuis, Marten, Rödel, Gerhard
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1262761/
https://www.ncbi.nlm.nih.gov/pubmed/16202167
http://dx.doi.org/10.1186/1475-2859-4-28