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Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells
BACKGROUND: Native as well as recombinant bacterial cell surface layer (S-layer) protein of Geobacillus (G.) stearothermophilus ATCC 12980 assembles to supramolecular structures with an oblique symmetry. Upon expression in E. coli, S-layer self assembly products are formed in the cytosol. We tested...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1262761/ https://www.ncbi.nlm.nih.gov/pubmed/16202167 http://dx.doi.org/10.1186/1475-2859-4-28 |