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Specific function of a plastid sigma factor for ndhF gene transcription

The complexity of the plastid transcriptional apparatus (two or three different RNA polymerases and numerous regulatory proteins) makes it very difficult to attribute specific function(s) to its individual components. We have characterized an Arabidopsis T-DNA insertion line disrupting the nuclear g...

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Autores principales: Favory, Jean-Jacques, Kobayshi, Masanori, Tanaka, Kan, Peltier, Gilles, Kreis, Martin, Valay, Jean-Gabriel, Lerbs-Mache, Silva
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1266065/
https://www.ncbi.nlm.nih.gov/pubmed/16243785
http://dx.doi.org/10.1093/nar/gki908
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author Favory, Jean-Jacques
Kobayshi, Masanori
Tanaka, Kan
Peltier, Gilles
Kreis, Martin
Valay, Jean-Gabriel
Lerbs-Mache, Silva
author_facet Favory, Jean-Jacques
Kobayshi, Masanori
Tanaka, Kan
Peltier, Gilles
Kreis, Martin
Valay, Jean-Gabriel
Lerbs-Mache, Silva
author_sort Favory, Jean-Jacques
collection PubMed
description The complexity of the plastid transcriptional apparatus (two or three different RNA polymerases and numerous regulatory proteins) makes it very difficult to attribute specific function(s) to its individual components. We have characterized an Arabidopsis T-DNA insertion line disrupting the nuclear gene coding for one of the six plastid sigma factors (SIG4) that regulate the activity of the plastid-encoded RNA polymerase PEP. This mutant shows a specific diminution of transcription of the plastid ndhF gene, coding for a subunit of the plastid NDH [NAD(P)H dehydrogenase] complex. The absence of another NDH subunit, i.e. NDHH, and the absence of a chlorophyll fluorescence transient previously attributed to the activity of the plastid NDH complex indicate a strong down-regulation of NDH activity in the mutant plants. Results suggest that plastid NDH activity is regulated on the transcriptional level by an ndhF-specific plastid sigma factor, SIG4.
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spelling pubmed-12660652005-10-28 Specific function of a plastid sigma factor for ndhF gene transcription Favory, Jean-Jacques Kobayshi, Masanori Tanaka, Kan Peltier, Gilles Kreis, Martin Valay, Jean-Gabriel Lerbs-Mache, Silva Nucleic Acids Res Article The complexity of the plastid transcriptional apparatus (two or three different RNA polymerases and numerous regulatory proteins) makes it very difficult to attribute specific function(s) to its individual components. We have characterized an Arabidopsis T-DNA insertion line disrupting the nuclear gene coding for one of the six plastid sigma factors (SIG4) that regulate the activity of the plastid-encoded RNA polymerase PEP. This mutant shows a specific diminution of transcription of the plastid ndhF gene, coding for a subunit of the plastid NDH [NAD(P)H dehydrogenase] complex. The absence of another NDH subunit, i.e. NDHH, and the absence of a chlorophyll fluorescence transient previously attributed to the activity of the plastid NDH complex indicate a strong down-regulation of NDH activity in the mutant plants. Results suggest that plastid NDH activity is regulated on the transcriptional level by an ndhF-specific plastid sigma factor, SIG4. Oxford University Press 2005 2005-10-20 /pmc/articles/PMC1266065/ /pubmed/16243785 http://dx.doi.org/10.1093/nar/gki908 Text en © The Author 2005. Published by Oxford University Press. All rights reserved
spellingShingle Article
Favory, Jean-Jacques
Kobayshi, Masanori
Tanaka, Kan
Peltier, Gilles
Kreis, Martin
Valay, Jean-Gabriel
Lerbs-Mache, Silva
Specific function of a plastid sigma factor for ndhF gene transcription
title Specific function of a plastid sigma factor for ndhF gene transcription
title_full Specific function of a plastid sigma factor for ndhF gene transcription
title_fullStr Specific function of a plastid sigma factor for ndhF gene transcription
title_full_unstemmed Specific function of a plastid sigma factor for ndhF gene transcription
title_short Specific function of a plastid sigma factor for ndhF gene transcription
title_sort specific function of a plastid sigma factor for ndhf gene transcription
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1266065/
https://www.ncbi.nlm.nih.gov/pubmed/16243785
http://dx.doi.org/10.1093/nar/gki908
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