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ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro
Asbestos is a known carcinogen and co-carcinogen. It is a persisting risk in our daily life due to its use in building material as asbestos-cement powder. The present study done on V79-cells (Chinese hamster lung cells) demonstrates the cytotoxic and genotoxic potential of asbestos-cement powder (AC...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1274344/ https://www.ncbi.nlm.nih.gov/pubmed/16209709 http://dx.doi.org/10.1186/1743-8977-2-9 |
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author | Dopp, Elke Yadav, Santosh Ansari, Furquan Ahmad Bhattacharya, Kunal von Recklinghausen, Ursula Rauen, Ursula Rödelsperger, Klaus Shokouhi, Behnaz Geh, Stefan Rahman, Qamar |
author_facet | Dopp, Elke Yadav, Santosh Ansari, Furquan Ahmad Bhattacharya, Kunal von Recklinghausen, Ursula Rauen, Ursula Rödelsperger, Klaus Shokouhi, Behnaz Geh, Stefan Rahman, Qamar |
author_sort | Dopp, Elke |
collection | PubMed |
description | Asbestos is a known carcinogen and co-carcinogen. It is a persisting risk in our daily life due to its use in building material as asbestos-cement powder. The present study done on V79-cells (Chinese hamster lung cells) demonstrates the cytotoxic and genotoxic potential of asbestos-cement powder (ACP) in comparison with chrysotile asbestos. A co-exposure of chrysotile and ACP was tested using the cell viability test and the micronucleus assay. The kinetochore analysis had been used to analyse the pathway causing such genotoxic effects. Thiobarbituric acid-reactive substances were determined as evidence for the production of reactive oxygen species. Both, asbestos cement as well as chrysotile formed micronuclei and induced loss of cell viability in a concentration- and time- dependent way. Results of TBARS analysis and iron chelator experiments showed induction of free radicals in ACP- and chrysotile exposed cultures. CaSO(4 )appeared to be a negligible entity in enhancing the toxic potential of ACP. The co-exposure of both, ACP and chrysotile, showed an additive effect in enhancing the toxicity. The overall study suggests that asbestos-cement is cytotoxic as well as genotoxic in vitro. In comparison to chrysotile the magnitude of the toxicity was less, but co-exposure increased the toxicity of both. |
format | Text |
id | pubmed-1274344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-12743442005-10-29 ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro Dopp, Elke Yadav, Santosh Ansari, Furquan Ahmad Bhattacharya, Kunal von Recklinghausen, Ursula Rauen, Ursula Rödelsperger, Klaus Shokouhi, Behnaz Geh, Stefan Rahman, Qamar Part Fibre Toxicol Research Asbestos is a known carcinogen and co-carcinogen. It is a persisting risk in our daily life due to its use in building material as asbestos-cement powder. The present study done on V79-cells (Chinese hamster lung cells) demonstrates the cytotoxic and genotoxic potential of asbestos-cement powder (ACP) in comparison with chrysotile asbestos. A co-exposure of chrysotile and ACP was tested using the cell viability test and the micronucleus assay. The kinetochore analysis had been used to analyse the pathway causing such genotoxic effects. Thiobarbituric acid-reactive substances were determined as evidence for the production of reactive oxygen species. Both, asbestos cement as well as chrysotile formed micronuclei and induced loss of cell viability in a concentration- and time- dependent way. Results of TBARS analysis and iron chelator experiments showed induction of free radicals in ACP- and chrysotile exposed cultures. CaSO(4 )appeared to be a negligible entity in enhancing the toxic potential of ACP. The co-exposure of both, ACP and chrysotile, showed an additive effect in enhancing the toxicity. The overall study suggests that asbestos-cement is cytotoxic as well as genotoxic in vitro. In comparison to chrysotile the magnitude of the toxicity was less, but co-exposure increased the toxicity of both. BioMed Central 2005-10-06 /pmc/articles/PMC1274344/ /pubmed/16209709 http://dx.doi.org/10.1186/1743-8977-2-9 Text en Copyright © 2005 Dopp et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Dopp, Elke Yadav, Santosh Ansari, Furquan Ahmad Bhattacharya, Kunal von Recklinghausen, Ursula Rauen, Ursula Rödelsperger, Klaus Shokouhi, Behnaz Geh, Stefan Rahman, Qamar ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro |
title | ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro |
title_full | ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro |
title_fullStr | ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro |
title_full_unstemmed | ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro |
title_short | ROS-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro |
title_sort | ros-mediated genotoxicity of asbestos-cement in mammalian lung cells in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1274344/ https://www.ncbi.nlm.nih.gov/pubmed/16209709 http://dx.doi.org/10.1186/1743-8977-2-9 |
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