Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat

Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transforma...

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Detalles Bibliográficos
Autores principales: Jones, Huw D, Doherty, Angela, Wu, Huixia
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1277018/
https://www.ncbi.nlm.nih.gov/pubmed/16270934
http://dx.doi.org/10.1186/1746-4811-1-5
Descripción
Sumario:Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

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