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Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901
We report here the sequencing and analysis of the genome of the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901. This species is a model for studies of hydrogenogens, which are diverse bacteria and archaea that grow anaerobically utilizing carbon monoxide (CO) as their sole carbon so...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1287953/ https://www.ncbi.nlm.nih.gov/pubmed/16311624 http://dx.doi.org/10.1371/journal.pgen.0010065 |
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author | Wu, Martin Ren, Qinghu Durkin, A. Scott Daugherty, Sean C Brinkac, Lauren M Dodson, Robert J Madupu, Ramana Sullivan, Steven A Kolonay, James F Nelson, William C Tallon, Luke J Jones, Kristine M Ulrich, Luke E Gonzalez, Juan M Zhulin, Igor B Robb, Frank T Eisen, Jonathan A |
author_facet | Wu, Martin Ren, Qinghu Durkin, A. Scott Daugherty, Sean C Brinkac, Lauren M Dodson, Robert J Madupu, Ramana Sullivan, Steven A Kolonay, James F Nelson, William C Tallon, Luke J Jones, Kristine M Ulrich, Luke E Gonzalez, Juan M Zhulin, Igor B Robb, Frank T Eisen, Jonathan A |
author_sort | Wu, Martin |
collection | PubMed |
description | We report here the sequencing and analysis of the genome of the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901. This species is a model for studies of hydrogenogens, which are diverse bacteria and archaea that grow anaerobically utilizing carbon monoxide (CO) as their sole carbon source and water as an electron acceptor, producing carbon dioxide and hydrogen as waste products. Organisms that make use of CO do so through carbon monoxide dehydrogenase complexes. Remarkably, analysis of the genome of C. hydrogenoformans reveals the presence of at least five highly differentiated anaerobic carbon monoxide dehydrogenase complexes, which may in part explain how this species is able to grow so much more rapidly on CO than many other species. Analysis of the genome also has provided many general insights into the metabolism of this organism which should make it easier to use it as a source of biologically produced hydrogen gas. One surprising finding is the presence of many genes previously found only in sporulating species in the Firmicutes Phylum. Although this species is also a Firmicutes, it was not known to sporulate previously. Here we show that it does sporulate and because it is missing many of the genes involved in sporulation in other species, this organism may serve as a “minimal” model for sporulation studies. In addition, using phylogenetic profile analysis, we have identified many uncharacterized gene families found in all known sporulating Firmicutes, but not in any non-sporulating bacteria, including a sigma factor not known to be involved in sporulation previously. |
format | Text |
id | pubmed-1287953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-12879532005-11-25 Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 Wu, Martin Ren, Qinghu Durkin, A. Scott Daugherty, Sean C Brinkac, Lauren M Dodson, Robert J Madupu, Ramana Sullivan, Steven A Kolonay, James F Nelson, William C Tallon, Luke J Jones, Kristine M Ulrich, Luke E Gonzalez, Juan M Zhulin, Igor B Robb, Frank T Eisen, Jonathan A PLoS Genet Research Article We report here the sequencing and analysis of the genome of the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901. This species is a model for studies of hydrogenogens, which are diverse bacteria and archaea that grow anaerobically utilizing carbon monoxide (CO) as their sole carbon source and water as an electron acceptor, producing carbon dioxide and hydrogen as waste products. Organisms that make use of CO do so through carbon monoxide dehydrogenase complexes. Remarkably, analysis of the genome of C. hydrogenoformans reveals the presence of at least five highly differentiated anaerobic carbon monoxide dehydrogenase complexes, which may in part explain how this species is able to grow so much more rapidly on CO than many other species. Analysis of the genome also has provided many general insights into the metabolism of this organism which should make it easier to use it as a source of biologically produced hydrogen gas. One surprising finding is the presence of many genes previously found only in sporulating species in the Firmicutes Phylum. Although this species is also a Firmicutes, it was not known to sporulate previously. Here we show that it does sporulate and because it is missing many of the genes involved in sporulation in other species, this organism may serve as a “minimal” model for sporulation studies. In addition, using phylogenetic profile analysis, we have identified many uncharacterized gene families found in all known sporulating Firmicutes, but not in any non-sporulating bacteria, including a sigma factor not known to be involved in sporulation previously. Public Library of Science 2005-11 2005-11-25 /pmc/articles/PMC1287953/ /pubmed/16311624 http://dx.doi.org/10.1371/journal.pgen.0010065 Text en Copyright: © 2005 Wu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wu, Martin Ren, Qinghu Durkin, A. Scott Daugherty, Sean C Brinkac, Lauren M Dodson, Robert J Madupu, Ramana Sullivan, Steven A Kolonay, James F Nelson, William C Tallon, Luke J Jones, Kristine M Ulrich, Luke E Gonzalez, Juan M Zhulin, Igor B Robb, Frank T Eisen, Jonathan A Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 |
title | Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 |
title_full | Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 |
title_fullStr | Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 |
title_full_unstemmed | Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 |
title_short | Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 |
title_sort | life in hot carbon monoxide: the complete genome sequence of carboxydothermus hydrogenoformans z-2901 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1287953/ https://www.ncbi.nlm.nih.gov/pubmed/16311624 http://dx.doi.org/10.1371/journal.pgen.0010065 |
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