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Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis

BACKGROUND: Reproductive systems of male moths contain circadian clocks, which time the release of sperm bundles from the testis to the upper vas deferens (UVD) and their subsequent transfer from the UVD to the seminal vesicles. Sperm bundles are released from the testis in the evening and are retai...

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Autores principales: Bebas, P, Maksimiuk, E, Gvakharia, B, Cymborowski, B, Giebultowicz, JM
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC128818/
https://www.ncbi.nlm.nih.gov/pubmed/12230632
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author Bebas, P
Maksimiuk, E
Gvakharia, B
Cymborowski, B
Giebultowicz, JM
author_facet Bebas, P
Maksimiuk, E
Gvakharia, B
Cymborowski, B
Giebultowicz, JM
author_sort Bebas, P
collection PubMed
description BACKGROUND: Reproductive systems of male moths contain circadian clocks, which time the release of sperm bundles from the testis to the upper vas deferens (UVD) and their subsequent transfer from the UVD to the seminal vesicles. Sperm bundles are released from the testis in the evening and are retained in the vas deferens lumen overnight before being transferred to the seminal vesicles. The biological significance of periodic sperm retention in the UVD lumen is not understood. In this study we asked whether there are circadian rhythms in the UVD that are correlated with sperm retention. RESULTS: We investigated the carbohydrate-rich material present in the UVD wall and lumen during the daily cycle of sperm release using the periodic acid-Shiff reaction (PAS). Males raised in 16:8 light-dark cycles (LD) showed a clear rhythm in the levels of PAS-positive granules in the apical portion of the UVD epithelium. The peak of granule accumulation occurred in the middle of the night and coincided with the maximum presence of sperm bundles in the UVD lumen. These rhythms persisted in constant darkness (DD), indicating that they have circadian nature. They were abolished, however, in constant light (LL) resulting in random patterns of PAS-positive material in the UVD wall. Gel-separation of the UVD homogenates from LD moths followed by detection of carbohydrates on blots revealed daily rhythms in the abundance of specific glycoproteins in the wall and lumen of the UVD. CONCLUSION: Secretory activity of the vas deferens epithelium is regulated by the circadian clock. Daily rhythms in accumulation and secretion of several glycoproteins are co-ordinated with periodic retention of sperm in the vas deferens lumen.
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spelling pubmed-1288182002-10-23 Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis Bebas, P Maksimiuk, E Gvakharia, B Cymborowski, B Giebultowicz, JM BMC Physiol Research Article BACKGROUND: Reproductive systems of male moths contain circadian clocks, which time the release of sperm bundles from the testis to the upper vas deferens (UVD) and their subsequent transfer from the UVD to the seminal vesicles. Sperm bundles are released from the testis in the evening and are retained in the vas deferens lumen overnight before being transferred to the seminal vesicles. The biological significance of periodic sperm retention in the UVD lumen is not understood. In this study we asked whether there are circadian rhythms in the UVD that are correlated with sperm retention. RESULTS: We investigated the carbohydrate-rich material present in the UVD wall and lumen during the daily cycle of sperm release using the periodic acid-Shiff reaction (PAS). Males raised in 16:8 light-dark cycles (LD) showed a clear rhythm in the levels of PAS-positive granules in the apical portion of the UVD epithelium. The peak of granule accumulation occurred in the middle of the night and coincided with the maximum presence of sperm bundles in the UVD lumen. These rhythms persisted in constant darkness (DD), indicating that they have circadian nature. They were abolished, however, in constant light (LL) resulting in random patterns of PAS-positive material in the UVD wall. Gel-separation of the UVD homogenates from LD moths followed by detection of carbohydrates on blots revealed daily rhythms in the abundance of specific glycoproteins in the wall and lumen of the UVD. CONCLUSION: Secretory activity of the vas deferens epithelium is regulated by the circadian clock. Daily rhythms in accumulation and secretion of several glycoproteins are co-ordinated with periodic retention of sperm in the vas deferens lumen. BioMed Central 2002-09-13 /pmc/articles/PMC128818/ /pubmed/12230632 Text en Copyright © 2002 Bebas et al; licensee BioMed Central Ltd. This article is published in Open Access: verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Bebas, P
Maksimiuk, E
Gvakharia, B
Cymborowski, B
Giebultowicz, JM
Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis
title Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis
title_full Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis
title_fullStr Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis
title_full_unstemmed Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis
title_short Circadian rhythm of glycoprotein secretion in the vas deferens of the moth, Spodoptera littoralis
title_sort circadian rhythm of glycoprotein secretion in the vas deferens of the moth, spodoptera littoralis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC128818/
https://www.ncbi.nlm.nih.gov/pubmed/12230632
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