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Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection
BACKGROUND: Constitutive expression and localization of antimicrobial human β-defensin-1 (HBD-1) in human kidneys as a potential mechanism of antimicrobial defense has been previously reported. Inducible expression of human β-defensin-2 (HBD-2) has been described in various epithelial organs but not...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC128826/ https://www.ncbi.nlm.nih.gov/pubmed/12238953 http://dx.doi.org/10.1186/1471-2334-2-20 |
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author | Lehmann, Jan Retz, Margitta Harder, Jürgen Krams, Matthias Kellner, Udo Hartmann, Julia Hohgräwe, Kerstin Raffenberg, Uta Gerber, Martin Loch, Tillmann Weichert-Jacobsen, Klaus Stöckle, Michael |
author_facet | Lehmann, Jan Retz, Margitta Harder, Jürgen Krams, Matthias Kellner, Udo Hartmann, Julia Hohgräwe, Kerstin Raffenberg, Uta Gerber, Martin Loch, Tillmann Weichert-Jacobsen, Klaus Stöckle, Michael |
author_sort | Lehmann, Jan |
collection | PubMed |
description | BACKGROUND: Constitutive expression and localization of antimicrobial human β-defensin-1 (HBD-1) in human kidneys as a potential mechanism of antimicrobial defense has been previously reported. Inducible expression of human β-defensin-2 (HBD-2) has been described in various epithelial organs but not for the urogenital tract. METHODS: We investigated the gene- and protein expression of HBD-1 and HBD-2 by reverse transcriptase-polymerase chain reaction, and immunohistochemistry in 15 normal human kidney samples and 15 renal tissues with chronic bacterial infection. Additionally, cell culture experiments were performed to study HBD gene expression by real-time RT-PCR in response to inflammatory cytokines TNFα and IL-1β as well as lipopolysaccharide from Gram-negative bacteria. RESULTS: Constitutive HBD-1 gene- and protein expression was detected in normal renal tissue and kidneys with chronic infection. As a novel finding, inducible HBD-2 gene- and protein expression was demonstrated in tubulus epithelia with chronic infection but not in normal renal tissue. In pyelonephritic kidneys HBD-1 and HBD-2 expression showed a similar pattern of localizaton in distal tubules, loops of Henle and in collecting ducts of the kidney. Furthermore, real-time RT-PCR of kidney derived cell lines stimulated with inflammatory agents TNF-α, IL-1β and LPS revealed a strong increase in relative HBD-2 transcription level and also a slight increase in relative HBD-1 transcription level. CONCLUSIONS: Upregulated HBD-2 expression in renal tubulus epithelium indicates a role of a wider range of human defensins for antimicrobial host defense in the urogenital tract than previously recognized. |
format | Text |
id | pubmed-128826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-1288262002-10-24 Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection Lehmann, Jan Retz, Margitta Harder, Jürgen Krams, Matthias Kellner, Udo Hartmann, Julia Hohgräwe, Kerstin Raffenberg, Uta Gerber, Martin Loch, Tillmann Weichert-Jacobsen, Klaus Stöckle, Michael BMC Infect Dis Research Article BACKGROUND: Constitutive expression and localization of antimicrobial human β-defensin-1 (HBD-1) in human kidneys as a potential mechanism of antimicrobial defense has been previously reported. Inducible expression of human β-defensin-2 (HBD-2) has been described in various epithelial organs but not for the urogenital tract. METHODS: We investigated the gene- and protein expression of HBD-1 and HBD-2 by reverse transcriptase-polymerase chain reaction, and immunohistochemistry in 15 normal human kidney samples and 15 renal tissues with chronic bacterial infection. Additionally, cell culture experiments were performed to study HBD gene expression by real-time RT-PCR in response to inflammatory cytokines TNFα and IL-1β as well as lipopolysaccharide from Gram-negative bacteria. RESULTS: Constitutive HBD-1 gene- and protein expression was detected in normal renal tissue and kidneys with chronic infection. As a novel finding, inducible HBD-2 gene- and protein expression was demonstrated in tubulus epithelia with chronic infection but not in normal renal tissue. In pyelonephritic kidneys HBD-1 and HBD-2 expression showed a similar pattern of localizaton in distal tubules, loops of Henle and in collecting ducts of the kidney. Furthermore, real-time RT-PCR of kidney derived cell lines stimulated with inflammatory agents TNF-α, IL-1β and LPS revealed a strong increase in relative HBD-2 transcription level and also a slight increase in relative HBD-1 transcription level. CONCLUSIONS: Upregulated HBD-2 expression in renal tubulus epithelium indicates a role of a wider range of human defensins for antimicrobial host defense in the urogenital tract than previously recognized. BioMed Central 2002-09-18 /pmc/articles/PMC128826/ /pubmed/12238953 http://dx.doi.org/10.1186/1471-2334-2-20 Text en Copyright © 2002 Lehmann et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Lehmann, Jan Retz, Margitta Harder, Jürgen Krams, Matthias Kellner, Udo Hartmann, Julia Hohgräwe, Kerstin Raffenberg, Uta Gerber, Martin Loch, Tillmann Weichert-Jacobsen, Klaus Stöckle, Michael Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection |
title | Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection |
title_full | Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection |
title_fullStr | Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection |
title_full_unstemmed | Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection |
title_short | Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection |
title_sort | expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC128826/ https://www.ncbi.nlm.nih.gov/pubmed/12238953 http://dx.doi.org/10.1186/1471-2334-2-20 |
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