A computational investigation of kinetoplastid trans-splicing

Trans-splicing is an unusual process in which two separate RNA strands are spliced together to yield a mature mRNA. We present a novel computational approach which has an overall accuracy of 82% and can predict 92% of known trans-splicing sites. We have applied our method to chromosomes 1 and 3 of L...

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Detalles Bibliográficos
Autores principales: Gopal, Shuba, Awadalla, Saria, Gaasterland, Terry, Cross, George AM
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1297651/
https://www.ncbi.nlm.nih.gov/pubmed/16277750
http://dx.doi.org/10.1186/gb-2005-6-11-r95
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author Gopal, Shuba
Awadalla, Saria
Gaasterland, Terry
Cross, George AM
author_facet Gopal, Shuba
Awadalla, Saria
Gaasterland, Terry
Cross, George AM
author_sort Gopal, Shuba
collection PubMed
description Trans-splicing is an unusual process in which two separate RNA strands are spliced together to yield a mature mRNA. We present a novel computational approach which has an overall accuracy of 82% and can predict 92% of known trans-splicing sites. We have applied our method to chromosomes 1 and 3 of Leishmania major, with high-confidence predictions for 85% and 88% of annotated genes respectively. We suggest some extensions of our method to other systems.
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spelling pubmed-12976512005-12-01 A computational investigation of kinetoplastid trans-splicing Gopal, Shuba Awadalla, Saria Gaasterland, Terry Cross, George AM Genome Biol Method Trans-splicing is an unusual process in which two separate RNA strands are spliced together to yield a mature mRNA. We present a novel computational approach which has an overall accuracy of 82% and can predict 92% of known trans-splicing sites. We have applied our method to chromosomes 1 and 3 of Leishmania major, with high-confidence predictions for 85% and 88% of annotated genes respectively. We suggest some extensions of our method to other systems. BioMed Central 2005 2005-10-17 /pmc/articles/PMC1297651/ /pubmed/16277750 http://dx.doi.org/10.1186/gb-2005-6-11-r95 Text en Copyright © 2005 Gopal et al.; licensee BioMed Central Ltd.
spellingShingle Method
Gopal, Shuba
Awadalla, Saria
Gaasterland, Terry
Cross, George AM
A computational investigation of kinetoplastid trans-splicing
title A computational investigation of kinetoplastid trans-splicing
title_full A computational investigation of kinetoplastid trans-splicing
title_fullStr A computational investigation of kinetoplastid trans-splicing
title_full_unstemmed A computational investigation of kinetoplastid trans-splicing
title_short A computational investigation of kinetoplastid trans-splicing
title_sort computational investigation of kinetoplastid trans-splicing
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1297651/
https://www.ncbi.nlm.nih.gov/pubmed/16277750
http://dx.doi.org/10.1186/gb-2005-6-11-r95
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