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A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices

BACKGROUND: Propagated tissue degeneration, especially during aging, has been shown to be enhanced through potentiation of innate immune responses. Neurodegenerative diseases and a wide variety of inflammatory conditions are linked together and several anti-inflammatory compounds considered as havin...

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Autores principales: Huuskonen, Jari, Suuronen, Tiina, Miettinen, Riitta, van Groen, Thomas, Salminen, Antero
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298326/
https://www.ncbi.nlm.nih.gov/pubmed/16285888
http://dx.doi.org/10.1186/1742-2094-2-25
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author Huuskonen, Jari
Suuronen, Tiina
Miettinen, Riitta
van Groen, Thomas
Salminen, Antero
author_facet Huuskonen, Jari
Suuronen, Tiina
Miettinen, Riitta
van Groen, Thomas
Salminen, Antero
author_sort Huuskonen, Jari
collection PubMed
description BACKGROUND: Propagated tissue degeneration, especially during aging, has been shown to be enhanced through potentiation of innate immune responses. Neurodegenerative diseases and a wide variety of inflammatory conditions are linked together and several anti-inflammatory compounds considered as having therapeutic potential for example in Alzheimer's disease (AD). In vitro brain slice techniques have been widely used to unravel the complexity of neuroinflammation, but rarely, has the power of the model itself been reported. Our aim was to gain a more detailed insight and understanding of the behaviour of hippocampus tissue slices in serum-free, interface culture per se and after exposure to different pro- and anti-inflammatory compounds. METHODS: The responses of the slices to pro- and anti-inflammatory stimuli were monitored at various time points by measuring the leakage of lactate dehydrogenase (LDH) and the release of cytokines interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF-α) and nitric oxide (NO) from the culture media. Histological methods were applied to reveal the morphological status after exposure to stimuli and during the time course of the culture period. Statistical power analysis were made with nQuery Advisor(®), version 5.0, (Statistical Solutions, Saugus, MA) computer program for Wilcoxon (Mann-Whitney) rank-sum test. RESULTS: By using the interface membrane culture technique, the hippocampal slices largely recover from the trauma caused by cutting after 4–5 days in vitro. Furthermore, the cultures remain stable and retain their responsiveness to inflammatory stimuli for at least 3 weeks. During this time period, cultures are susceptible to modification by inflammatory stimuli as assessed by quantitative biochemical assays and morphological characterizations. CONCLUSION: The present report outlines the techniques for studying immune responses using a serum-free slice culture model. Statistically powerful data under controlled culture conditions and with ethically justified use of animals can be obtained as soon as after 4–5 DIV. The model is most probably suitable also for studies of chronic inflammation.
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spelling pubmed-12983262005-12-02 A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices Huuskonen, Jari Suuronen, Tiina Miettinen, Riitta van Groen, Thomas Salminen, Antero J Neuroinflammation Research BACKGROUND: Propagated tissue degeneration, especially during aging, has been shown to be enhanced through potentiation of innate immune responses. Neurodegenerative diseases and a wide variety of inflammatory conditions are linked together and several anti-inflammatory compounds considered as having therapeutic potential for example in Alzheimer's disease (AD). In vitro brain slice techniques have been widely used to unravel the complexity of neuroinflammation, but rarely, has the power of the model itself been reported. Our aim was to gain a more detailed insight and understanding of the behaviour of hippocampus tissue slices in serum-free, interface culture per se and after exposure to different pro- and anti-inflammatory compounds. METHODS: The responses of the slices to pro- and anti-inflammatory stimuli were monitored at various time points by measuring the leakage of lactate dehydrogenase (LDH) and the release of cytokines interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF-α) and nitric oxide (NO) from the culture media. Histological methods were applied to reveal the morphological status after exposure to stimuli and during the time course of the culture period. Statistical power analysis were made with nQuery Advisor(®), version 5.0, (Statistical Solutions, Saugus, MA) computer program for Wilcoxon (Mann-Whitney) rank-sum test. RESULTS: By using the interface membrane culture technique, the hippocampal slices largely recover from the trauma caused by cutting after 4–5 days in vitro. Furthermore, the cultures remain stable and retain their responsiveness to inflammatory stimuli for at least 3 weeks. During this time period, cultures are susceptible to modification by inflammatory stimuli as assessed by quantitative biochemical assays and morphological characterizations. CONCLUSION: The present report outlines the techniques for studying immune responses using a serum-free slice culture model. Statistically powerful data under controlled culture conditions and with ethically justified use of animals can be obtained as soon as after 4–5 DIV. The model is most probably suitable also for studies of chronic inflammation. BioMed Central 2005-11-15 /pmc/articles/PMC1298326/ /pubmed/16285888 http://dx.doi.org/10.1186/1742-2094-2-25 Text en Copyright © 2005 Huuskonen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Huuskonen, Jari
Suuronen, Tiina
Miettinen, Riitta
van Groen, Thomas
Salminen, Antero
A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
title A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
title_full A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
title_fullStr A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
title_full_unstemmed A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
title_short A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
title_sort refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298326/
https://www.ncbi.nlm.nih.gov/pubmed/16285888
http://dx.doi.org/10.1186/1742-2094-2-25
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