Cargando…
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
This study addresses the problem of PCR inhibition by reverse transcriptase. It has been shown that the inhibition occurs mostly when a small amount of RNA is taken for RT reaction, and it is more visible for rarely expressed transcripts. We show here that the inhibition takes place regardless of wh...
| Autores principales: | , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298932/ https://www.ncbi.nlm.nih.gov/pubmed/16314311 http://dx.doi.org/10.1093/nar/gni176 |