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Cross-species hybridisation of pig RNA to human nylon microarrays

BACKGROUND: The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon micro...

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Autores principales: Moody, DE, Zou, Z, McIntyre, L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC130049/
https://www.ncbi.nlm.nih.gov/pubmed/12354330
http://dx.doi.org/10.1186/1471-2164-3-27
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author Moody, DE
Zou, Z
McIntyre, L
author_facet Moody, DE
Zou, Z
McIntyre, L
author_sort Moody, DE
collection PubMed
description BACKGROUND: The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon microarrays were completed. Three replicate hybridisations of two different amounts of pig RNA, and of human skeletal muscle RNA were completed on three additional microarrays. RESULTS: Reproducibility of microarray hybridisations of pig cDNA to human microarrays was high, as determined by Spearman and Pearson correlation coefficients and a Kappa statistic. Variability among replicate hybridisations was similar for human and pig data, indicating the reproducibility of results were not compromised in cross-species hybridisations. The concordance between data generated from hybridisations using pig and human skeletal muscle RNA was high, further supporting the use of human microarrays for the analysis of gene expression in the pig. No systematic effect of stripping and re-using nylon microarrays was found, and variability across microarrays was minimal. CONCLUSION: The majority of genes generated highly reproducible data in cross-species microarray hybridisations, although approximately 6% were identified as highly variable. Experimental designs that include at least three replicate hybridisations for each experimental treatment will enable the variability of individual genes to be considered appropriately. The use of cross-species microarray analysis looks promising. However, additional validation is needed to determine the specificity of cross-species hybridisations, and the validity of results.
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spelling pubmed-1300492002-10-25 Cross-species hybridisation of pig RNA to human nylon microarrays Moody, DE Zou, Z McIntyre, L BMC Genomics Research Article BACKGROUND: The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon microarrays were completed. Three replicate hybridisations of two different amounts of pig RNA, and of human skeletal muscle RNA were completed on three additional microarrays. RESULTS: Reproducibility of microarray hybridisations of pig cDNA to human microarrays was high, as determined by Spearman and Pearson correlation coefficients and a Kappa statistic. Variability among replicate hybridisations was similar for human and pig data, indicating the reproducibility of results were not compromised in cross-species hybridisations. The concordance between data generated from hybridisations using pig and human skeletal muscle RNA was high, further supporting the use of human microarrays for the analysis of gene expression in the pig. No systematic effect of stripping and re-using nylon microarrays was found, and variability across microarrays was minimal. CONCLUSION: The majority of genes generated highly reproducible data in cross-species microarray hybridisations, although approximately 6% were identified as highly variable. Experimental designs that include at least three replicate hybridisations for each experimental treatment will enable the variability of individual genes to be considered appropriately. The use of cross-species microarray analysis looks promising. However, additional validation is needed to determine the specificity of cross-species hybridisations, and the validity of results. BioMed Central 2002-09-27 /pmc/articles/PMC130049/ /pubmed/12354330 http://dx.doi.org/10.1186/1471-2164-3-27 Text en Copyright © 2002 Moody et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Moody, DE
Zou, Z
McIntyre, L
Cross-species hybridisation of pig RNA to human nylon microarrays
title Cross-species hybridisation of pig RNA to human nylon microarrays
title_full Cross-species hybridisation of pig RNA to human nylon microarrays
title_fullStr Cross-species hybridisation of pig RNA to human nylon microarrays
title_full_unstemmed Cross-species hybridisation of pig RNA to human nylon microarrays
title_short Cross-species hybridisation of pig RNA to human nylon microarrays
title_sort cross-species hybridisation of pig rna to human nylon microarrays
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC130049/
https://www.ncbi.nlm.nih.gov/pubmed/12354330
http://dx.doi.org/10.1186/1471-2164-3-27
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