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Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study

BACKGROUND: Formulae for infants with cow's milk protein allergy (CMA) should be based on extensively hydrolysed protein. 'Extensively' however is not strictly defined. Differences in molecular weight and peptide chain length may affect its clinical outcome. We studied the safety of a...

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Autores principales: Terheggen-Lagro, Suzanne WJ, Khouw, Ilse MSL, Schaafsma, Anne, Wauters, Erik AK
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC130212/
https://www.ncbi.nlm.nih.gov/pubmed/12379156
http://dx.doi.org/10.1186/1471-2431-2-10
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author Terheggen-Lagro, Suzanne WJ
Khouw, Ilse MSL
Schaafsma, Anne
Wauters, Erik AK
author_facet Terheggen-Lagro, Suzanne WJ
Khouw, Ilse MSL
Schaafsma, Anne
Wauters, Erik AK
author_sort Terheggen-Lagro, Suzanne WJ
collection PubMed
description BACKGROUND: Formulae for infants with cow's milk protein allergy (CMA) should be based on extensively hydrolysed protein. 'Extensively' however is not strictly defined. Differences in molecular weight and peptide chain length may affect its clinical outcome. We studied the safety of a new extensively hydrolysed casein based formula (Frisolac Allergycare(®): FAC) for children with IgE mediated CMA. METHODS: Thirty children, aged 1.5 – 14.8 years old (median 4.9 years) with persistent CMA were enrolled in this double-blind reference product (Nutramigen(®): NUT) controlled crossover study. All had positive skin prick tests (SPT) and IgE mediated allergy, showing immediate reactions after ingestion of small amounts of milk. Twenty-five children also had positive radio allergen sorbent tests (RAST) to cow's milk. Formulae provided consisted of 80% elementary formula in combination with 20% reference or test product. Crossover periods lasted for two weeks. From both products molecular weight (MALDI-TOF method and HPLC) and peptide chain length distribution (adapted Edman degradation) were determined. RESULTS: Maximum molecular weights of NUT and FAC are 2.1 and 2.56 kDa, respectively. The contribution of free amino acids and small peptides <0.5 kDa is 46% for FAC and 53% for NUT. About 50% of the protein fraction of both products consists of peptides longer than four amino acids. Three children did not complete the study. The other children all tolerated FAC very well; no adverse reactions were reported. CONCLUSIONS: The new extensively hydrolysed casein-based formula (FAC) can safely be used in children with IgE mediated cow's milk allergy.
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spelling pubmed-1302122002-11-01 Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study Terheggen-Lagro, Suzanne WJ Khouw, Ilse MSL Schaafsma, Anne Wauters, Erik AK BMC Pediatr Research Article BACKGROUND: Formulae for infants with cow's milk protein allergy (CMA) should be based on extensively hydrolysed protein. 'Extensively' however is not strictly defined. Differences in molecular weight and peptide chain length may affect its clinical outcome. We studied the safety of a new extensively hydrolysed casein based formula (Frisolac Allergycare(®): FAC) for children with IgE mediated CMA. METHODS: Thirty children, aged 1.5 – 14.8 years old (median 4.9 years) with persistent CMA were enrolled in this double-blind reference product (Nutramigen(®): NUT) controlled crossover study. All had positive skin prick tests (SPT) and IgE mediated allergy, showing immediate reactions after ingestion of small amounts of milk. Twenty-five children also had positive radio allergen sorbent tests (RAST) to cow's milk. Formulae provided consisted of 80% elementary formula in combination with 20% reference or test product. Crossover periods lasted for two weeks. From both products molecular weight (MALDI-TOF method and HPLC) and peptide chain length distribution (adapted Edman degradation) were determined. RESULTS: Maximum molecular weights of NUT and FAC are 2.1 and 2.56 kDa, respectively. The contribution of free amino acids and small peptides <0.5 kDa is 46% for FAC and 53% for NUT. About 50% of the protein fraction of both products consists of peptides longer than four amino acids. Three children did not complete the study. The other children all tolerated FAC very well; no adverse reactions were reported. CONCLUSIONS: The new extensively hydrolysed casein-based formula (FAC) can safely be used in children with IgE mediated cow's milk allergy. BioMed Central 2002-10-14 /pmc/articles/PMC130212/ /pubmed/12379156 http://dx.doi.org/10.1186/1471-2431-2-10 Text en Copyright © 2002 Terheggen-Lagro et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Terheggen-Lagro, Suzanne WJ
Khouw, Ilse MSL
Schaafsma, Anne
Wauters, Erik AK
Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study
title Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study
title_full Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study
title_fullStr Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study
title_full_unstemmed Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study
title_short Safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study
title_sort safety of a new extensively hydrolysed formula in children with cow's milk protein allergy: a double blind crossover study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC130212/
https://www.ncbi.nlm.nih.gov/pubmed/12379156
http://dx.doi.org/10.1186/1471-2431-2-10
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