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The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori
Bmdsx is an orthologue of the sex-determining gene doublesex (dsx) and known to be sex-specifically expressed in various tissues of the silkworm, Bombyx mori. Its pre-mRNA is sex-specifically spliced and encodes female-specific or male-specific polypeptides. The open reading frame of Bmdsx consists...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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University of Arizona Library
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1307578/ https://www.ncbi.nlm.nih.gov/pubmed/16341249 |
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author | Funaguma, Shunsuke Suzuki, Masataka G. Tamura, Toshiki Shimada, Toru |
author_facet | Funaguma, Shunsuke Suzuki, Masataka G. Tamura, Toshiki Shimada, Toru |
author_sort | Funaguma, Shunsuke |
collection | PubMed |
description | Bmdsx is an orthologue of the sex-determining gene doublesex (dsx) and known to be sex-specifically expressed in various tissues of the silkworm, Bombyx mori. Its pre-mRNA is sex-specifically spliced and encodes female-specific or male-specific polypeptides. The open reading frame of Bmdsx consists of 5 exons, of which exons 3 and 4 are female-specific and its pre-mRNA was known to undergo default processing to generate the female-type mRNA. Previous reports have shown that the mechanism of splicing of the doublesex gene is different in Drosophila melanogaster and Bombyx mori. However, intron 4 is so long that it is difficult to identify the intronic cis-element(s) required for male-specific splicing of Bmdsx pre-mRNA using Bmdsx minigenes whose introns are shortened in various manners. As a first step toward discovery of the cis-element, the Bmdsx mini gene, which consisted of exon 1 and 5 and internally shortened introns 2 to 4, was constructed, and transgenic silkworms expressing this construct were generated. Bmdsx pre-mRNA transcribed derived from transgene was sex-specifically spliced. This result shows that the mini gene contained the information necessary for the correct regulation of alternative splicing. |
format | Text |
id | pubmed-1307578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | University of Arizona Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-13075782005-12-07 The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori Funaguma, Shunsuke Suzuki, Masataka G. Tamura, Toshiki Shimada, Toru J Insect Sci Articles Bmdsx is an orthologue of the sex-determining gene doublesex (dsx) and known to be sex-specifically expressed in various tissues of the silkworm, Bombyx mori. Its pre-mRNA is sex-specifically spliced and encodes female-specific or male-specific polypeptides. The open reading frame of Bmdsx consists of 5 exons, of which exons 3 and 4 are female-specific and its pre-mRNA was known to undergo default processing to generate the female-type mRNA. Previous reports have shown that the mechanism of splicing of the doublesex gene is different in Drosophila melanogaster and Bombyx mori. However, intron 4 is so long that it is difficult to identify the intronic cis-element(s) required for male-specific splicing of Bmdsx pre-mRNA using Bmdsx minigenes whose introns are shortened in various manners. As a first step toward discovery of the cis-element, the Bmdsx mini gene, which consisted of exon 1 and 5 and internally shortened introns 2 to 4, was constructed, and transgenic silkworms expressing this construct were generated. Bmdsx pre-mRNA transcribed derived from transgene was sex-specifically spliced. This result shows that the mini gene contained the information necessary for the correct regulation of alternative splicing. University of Arizona Library 2005-05-25 /pmc/articles/PMC1307578/ /pubmed/16341249 Text en Copyright © 2005. Open access; copyright is maintained by the authors. |
spellingShingle | Articles Funaguma, Shunsuke Suzuki, Masataka G. Tamura, Toshiki Shimada, Toru The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori |
title | The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori |
title_full | The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori |
title_fullStr | The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori |
title_full_unstemmed | The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori |
title_short | The Bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, Bombyx mori |
title_sort | bmdsx transgene including trimmed introns is sex-specifically spliced in tissues of the silkworm, bombyx mori |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1307578/ https://www.ncbi.nlm.nih.gov/pubmed/16341249 |
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