A reference database for tumor-related genes co-expressed with interleukin-8 using genome-scale in silico analysis

BACKGROUND: The EST database provides a rich resource for gene discovery and in silico expression analysis. We report a novel computational approach to identify co-expressed genes using EST database, and its application to IL-8. RESULTS: IL-8 is represented in 53 dbEST cDNA libraries. We calculated...

Descripción completa

Detalles Bibliográficos
Autores principales: Benbow, Lawrence, Wang, Lynn, Laverty, Maureen, Liu, Suxing, Qiu, Ping, Bond, Richard W, Gustafson, Eric, Hedrick, Joseph A, Kostich, Mitchell, Greene, Jonathan R, Wang, Luquan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC131052/
https://www.ncbi.nlm.nih.gov/pubmed/12377104
http://dx.doi.org/10.1186/1471-2164-3-29
Descripción
Sumario:BACKGROUND: The EST database provides a rich resource for gene discovery and in silico expression analysis. We report a novel computational approach to identify co-expressed genes using EST database, and its application to IL-8. RESULTS: IL-8 is represented in 53 dbEST cDNA libraries. We calculated the frequency of occurrence of all the genes represented in these cDNA libraries, and ranked the candidates based on a Z-score. Additional analysis suggests that most IL-8 related genes are differentially expressed between non-tumor and tumor tissues. To focus on IL-8's function in tumor tissues, we further analyzed and ranked the genes in 16 IL-8 related tumor libraries. CONCLUSIONS: This method generated a reference database for genes co-expressed with IL-8 and could facilitate further characterization of functional association among genes.

Ejemplares similares