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Identification of Drosophila Gene Products Required for Phagocytosis of Candida albicans
Phagocytosis is a highly conserved aspect of innate immunity. We used Drosophila melanogaster S2 cells as a model system to study the phagocytosis of Candida albicans, the major fungal pathogen of humans, by screening an RNAi library representing 7,216 fly genes conserved among metazoans. After resc...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1310651/ https://www.ncbi.nlm.nih.gov/pubmed/16336044 http://dx.doi.org/10.1371/journal.pbio.0040004 |
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author | Stroschein-Stevenson, Shannon L Foley, Edan O'Farrell, Patrick H Johnson, Alexander D |
author_facet | Stroschein-Stevenson, Shannon L Foley, Edan O'Farrell, Patrick H Johnson, Alexander D |
author_sort | Stroschein-Stevenson, Shannon L |
collection | PubMed |
description | Phagocytosis is a highly conserved aspect of innate immunity. We used Drosophila melanogaster S2 cells as a model system to study the phagocytosis of Candida albicans, the major fungal pathogen of humans, by screening an RNAi library representing 7,216 fly genes conserved among metazoans. After rescreening the initial genes identified and eliminating certain classes of housekeeping genes, we identified 184 genes required for efficient phagocytosis of C. albicans. Diverse biological processes are represented, with actin cytoskeleton regulation, vesicle transport, signaling, and transcriptional regulation being prominent. Secondary screens using Escherichia coli and latex beads revealed several genes specific for C. albicans phagocytosis. Characterization of one of those gene products, Macroglobulin complement related (Mcr), shows that it is secreted, that it binds specifically to the surface of C. albicans, and that it promotes its subsequent phagocytosis. Mcr is closely related to the four Drosophila thioester proteins (Teps), and we show that TepII is required for efficient phagocytosis of E. coli (but not C. albicans or Staphylococcus aureus) and that TepIII is required for the efficient phagocytosis of S. aureus (but not C. albicans or E. coli). Thus, this family of fly proteins distinguishes different pathogens for subsequent phagocytosis. |
format | Text |
id | pubmed-1310651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-13106512005-12-20 Identification of Drosophila Gene Products Required for Phagocytosis of Candida albicans Stroschein-Stevenson, Shannon L Foley, Edan O'Farrell, Patrick H Johnson, Alexander D PLoS Biol Research Article Phagocytosis is a highly conserved aspect of innate immunity. We used Drosophila melanogaster S2 cells as a model system to study the phagocytosis of Candida albicans, the major fungal pathogen of humans, by screening an RNAi library representing 7,216 fly genes conserved among metazoans. After rescreening the initial genes identified and eliminating certain classes of housekeeping genes, we identified 184 genes required for efficient phagocytosis of C. albicans. Diverse biological processes are represented, with actin cytoskeleton regulation, vesicle transport, signaling, and transcriptional regulation being prominent. Secondary screens using Escherichia coli and latex beads revealed several genes specific for C. albicans phagocytosis. Characterization of one of those gene products, Macroglobulin complement related (Mcr), shows that it is secreted, that it binds specifically to the surface of C. albicans, and that it promotes its subsequent phagocytosis. Mcr is closely related to the four Drosophila thioester proteins (Teps), and we show that TepII is required for efficient phagocytosis of E. coli (but not C. albicans or Staphylococcus aureus) and that TepIII is required for the efficient phagocytosis of S. aureus (but not C. albicans or E. coli). Thus, this family of fly proteins distinguishes different pathogens for subsequent phagocytosis. Public Library of Science 2006-01 2005-12-20 /pmc/articles/PMC1310651/ /pubmed/16336044 http://dx.doi.org/10.1371/journal.pbio.0040004 Text en Copyright: © 2006 Stroschein-Stevenson et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Stroschein-Stevenson, Shannon L Foley, Edan O'Farrell, Patrick H Johnson, Alexander D Identification of Drosophila Gene Products Required for Phagocytosis of Candida albicans |
title | Identification of Drosophila Gene Products Required for Phagocytosis of Candida
albicans
|
title_full | Identification of Drosophila Gene Products Required for Phagocytosis of Candida
albicans
|
title_fullStr | Identification of Drosophila Gene Products Required for Phagocytosis of Candida
albicans
|
title_full_unstemmed | Identification of Drosophila Gene Products Required for Phagocytosis of Candida
albicans
|
title_short | Identification of Drosophila Gene Products Required for Phagocytosis of Candida
albicans
|
title_sort | identification of drosophila gene products required for phagocytosis of candida
albicans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1310651/ https://www.ncbi.nlm.nih.gov/pubmed/16336044 http://dx.doi.org/10.1371/journal.pbio.0040004 |
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