Cargando…

Iterative in vivo assembly of large and complex transgenes by combining the activities of φC31 integrase and Cre recombinase

We have used the φC31 integrase to introduce large DNA sequences into a vertebrate genome and measure the efficiency of integration of intact DNA as a function of insert size. Inserts of 110 kb and 140 kb in length may be integrated with about 25% and 10% efficiency respectively. In order to overcom...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dafhnis-Calas, Felix, Xu, Zhengyao, Haines, Steve, Malla, Sunir K., Smith, Margaret C. M., Brown, William R. A.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2005
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316120/ https://www.ncbi.nlm.nih.gov/pubmed/16361264 http://dx.doi.org/10.1093/nar/gni192

Ejemplares similares

Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage ϕBT1
por: Xu, Zhengyao, et al.
Publicado: (2008)

Rearranging the centromere of the human Y chromosome with φC31 integrase
por: Malla, Sunir, et al.
Publicado: (2005)

Regulated gene insertion by steroid-induced ΦC31 integrase
por: Sharma, Nynne, et al.
Publicado: (2008)

Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange
por: Wong, Ee Tsin, et al.
Publicado: (2005)

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
por: Khodakaramian, Gholam, et al.
Publicado: (2006)

Zinc is essential for high-affinity DNA binding and recombinase activity of φC31 integrase
por: McEwan, Andrew R., et al.
Publicado: (2011)

Flp and Cre expressed from Flp–2A–Cre and Flp–IRES–Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange
por: Anderson, Rachelle P., et al.
Publicado: (2012)

Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent
por: Meador, Kyle, et al.
Publicado: (2019)

In vivo evaluation of PhiC31 recombinase activity using a self-excision cassette
por: Sangiorgi, Eugenio, et al.
Publicado: (2008)

Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification
por: Cain-Hom, Carol, et al.
Publicado: (2017)

Cre reconstitution allows for DNA recombination selectively in dual-marker-expressing cells in transgenic mice
por: Xu, Yanwen, et al.
Publicado: (2007)

Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction
por: Belteki, Gusztav, et al.
Publicado: (2005)

Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination
por: Colloms, Sean D., et al.
Publicado: (2014)

The mechanism of ϕC31 integrase directionality: experimental analysis and computational modelling
por: Pokhilko, Alexandra, et al.
Publicado: (2016)

Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells
por: Zhu, Haibao, et al.
Publicado: (2013)

DAXX interacts with phage ΦC31 integrase and inhibits recombination
por: Chen, Jin-zhong, et al.
Publicado: (2006)

DNA binding and synapsis by the large C-terminal domain of ϕC31 integrase
por: McEwan, Andrew R., et al.
Publicado: (2009)

Integrase-directed recovery of functional genes from genomic libraries
por: Rowe-Magnus, Dean A.
Publicado: (2009)

Production of viral vectors using recombinase-mediated cassette exchange
por: Nakano, Masakazu, et al.
Publicado: (2005)

Selection of bacteriophage λ integrases with altered recombination specificity by in vitro compartmentalization
por: Tay, Yvonne, et al.
Publicado: (2010)

Cell type differences in activity of the Streptomyces bacteriophage ϕC31 integrase
por: Maucksch, Christof, et al.
Publicado: (2008)

A motif in the C-terminal domain of ϕC31 integrase controls the directionality of recombination
por: Rowley, Paul A., et al.
Publicado: (2008)

Recombinase mediated cassette exchange into genomic targets using an adenovirus vector
por: Sorrell, David A., et al.
Publicado: (2010)

Unsuccessful attempt at gene-editing by homologous recombination in the zebrafish germ line using the approach of “Rong and Golic”
por: Brookfield, Rosalind, et al.
Publicado: (2012)

Sequences in attB that affect the ability of ϕC31 integrase to synapse and to activate DNA cleavage
por: Gupta, Milind, et al.
Publicado: (2007)

Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases
por: Krishnakumar, Radha, et al.
Publicado: (2014)

rDNA-directed integration by an HIV-1 integrase—I-PpoI fusion protein
por: Schenkwein, Diana, et al.
Publicado: (2013)

A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression
por: Makhija, Harshyaa, et al.
Publicado: (2018)

Bayesian multiple-instance motif discovery with BAMBI: inference of recombinase and transcription factor binding sites
por: Jajamovich, Guido H., et al.
Publicado: (2011)

Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers
por: Briggs, Adrian W., et al.
Publicado: (2012)

Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination
por: Van Nieuwerburgh, Filip, et al.
Publicado: (2012)

The dynamics of genome replication using deep sequencing
por: Müller, Carolin A., et al.
Publicado: (2014)

Enhanced and prolonged baculovirus-mediated expression by incorporating recombinase system and in cis elements: a comparative study
por: Sung, Li-Yu, et al.
Publicado: (2013)

iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains
por: Gräwe, Alexander, et al.
Publicado: (2020)

Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference
por: Hitz, Christiane, et al.
Publicado: (2007)

Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA interference
por: Coumoul, Xavier, et al.
Publicado: (2005)

Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing
por: Olorunniji, Femi J, et al.
Publicado: (2019)

An iterative network partition algorithm for accurate identification of dense network modules
por: Sun, Siqi, et al.
Publicado: (2012)

Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors
por: Ramachandra, Chrishan J. A., et al.
Publicado: (2011)

Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation
por: Hastie, Alex R., et al.
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_st6d1rb6s9rnst4votosrdlob5