Cargando…

Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand

The resolution of the dimeric intermolecular G-quadruplex/duplex competition of the telomeric DNA sequence 5′-TAG GGT TAG GGT-3′ and of its complementary 5′ ACC CTA ACC CTA-3′ is reported. To achieve this goal, melting experiments of both sequences and of the mixtures of these sequences were monitor...

Descripción completa

Detalles Bibliográficos
Autores principales: Jaumot, Joaquim, Eritja, Ramon, Tauler, Romà, Gargallo, Raimundo
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1325204/
https://www.ncbi.nlm.nih.gov/pubmed/16397299
http://dx.doi.org/10.1093/nar/gkj421
_version_ 1782126470970212352
author Jaumot, Joaquim
Eritja, Ramon
Tauler, Romà
Gargallo, Raimundo
author_facet Jaumot, Joaquim
Eritja, Ramon
Tauler, Romà
Gargallo, Raimundo
author_sort Jaumot, Joaquim
collection PubMed
description The resolution of the dimeric intermolecular G-quadruplex/duplex competition of the telomeric DNA sequence 5′-TAG GGT TAG GGT-3′ and of its complementary 5′ ACC CTA ACC CTA-3′ is reported. To achieve this goal, melting experiments of both sequences and of the mixtures of these sequences were monitored by molecular absorption, molecular fluorescence and circular dichroism spectroscopies. Molecular fluorescence measurements were carried out using molecular beacons technology, in which the 5′-TAG GGT TAG GGT-3′ sequence was labelled with a fluorophore and a quencher at the ends of the strand. Mathematical analysis of experimental spectroscopic data was performed by means of multivariate curve resolution, allowing the calculation of concentration profiles and pure spectra of all resolved structures (dimeric antiparallel and parallel G-quadruplexes, Watson–Crick duplex and single strands) present in solution. Our results show that parallel G-quadruplex is more stable than antiparallel G-quadruplex. When the complementary C-rich strand is present, a mixture of both G-quadruplex structures and Watson–Crick duplex is observed, the duplex being the major species. In addition to melting temperatures, equilibrium constants for the parallel/antiparallel G-quadruplex equilibrium and for the G-quadruplex/duplex equilibrium were determined from the concentration profiles.
format Text
id pubmed-1325204
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-13252042006-01-10 Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand Jaumot, Joaquim Eritja, Ramon Tauler, Romà Gargallo, Raimundo Nucleic Acids Res Article The resolution of the dimeric intermolecular G-quadruplex/duplex competition of the telomeric DNA sequence 5′-TAG GGT TAG GGT-3′ and of its complementary 5′ ACC CTA ACC CTA-3′ is reported. To achieve this goal, melting experiments of both sequences and of the mixtures of these sequences were monitored by molecular absorption, molecular fluorescence and circular dichroism spectroscopies. Molecular fluorescence measurements were carried out using molecular beacons technology, in which the 5′-TAG GGT TAG GGT-3′ sequence was labelled with a fluorophore and a quencher at the ends of the strand. Mathematical analysis of experimental spectroscopic data was performed by means of multivariate curve resolution, allowing the calculation of concentration profiles and pure spectra of all resolved structures (dimeric antiparallel and parallel G-quadruplexes, Watson–Crick duplex and single strands) present in solution. Our results show that parallel G-quadruplex is more stable than antiparallel G-quadruplex. When the complementary C-rich strand is present, a mixture of both G-quadruplex structures and Watson–Crick duplex is observed, the duplex being the major species. In addition to melting temperatures, equilibrium constants for the parallel/antiparallel G-quadruplex equilibrium and for the G-quadruplex/duplex equilibrium were determined from the concentration profiles. Oxford University Press 2006 2006-01-05 /pmc/articles/PMC1325204/ /pubmed/16397299 http://dx.doi.org/10.1093/nar/gkj421 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Article
Jaumot, Joaquim
Eritja, Ramon
Tauler, Romà
Gargallo, Raimundo
Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand
title Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand
title_full Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand
title_fullStr Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand
title_full_unstemmed Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand
title_short Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand
title_sort resolution of a structural competition involving dimeric g-quadruplex and its c-rich complementary strand
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1325204/
https://www.ncbi.nlm.nih.gov/pubmed/16397299
http://dx.doi.org/10.1093/nar/gkj421
work_keys_str_mv AT jaumotjoaquim resolutionofastructuralcompetitioninvolvingdimericgquadruplexanditscrichcomplementarystrand
AT eritjaramon resolutionofastructuralcompetitioninvolvingdimericgquadruplexanditscrichcomplementarystrand
AT taulerroma resolutionofastructuralcompetitioninvolvingdimericgquadruplexanditscrichcomplementarystrand
AT gargalloraimundo resolutionofastructuralcompetitioninvolvingdimericgquadruplexanditscrichcomplementarystrand