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Selection of primer-template sequences that bind human immunodeficiency virus reverse transcriptase with high affinity

A SELEX (systematic evolution of ligands by exponential enrichment)-based approach was developed to determine whether HIV-RT showed preference for particular primer-template sequences. A 70 nt duplex DNA was designed with 20 nt fixed flanking sequences at the 3′ and 5′ ends and a randomized 30 nt in...

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Detalles Bibliográficos
Autores principales: DeStefano, Jeffrey J., Cristofaro, Jason V.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1325207/
https://www.ncbi.nlm.nih.gov/pubmed/16397296
http://dx.doi.org/10.1093/nar/gkj426