Cargando…

Selection of primer-template sequences that bind human immunodeficiency virus reverse transcriptase with high affinity

A SELEX (systematic evolution of ligands by exponential enrichment)-based approach was developed to determine whether HIV-RT showed preference for particular primer-template sequences. A 70 nt duplex DNA was designed with 20 nt fixed flanking sequences at the 3′ and 5′ ends and a randomized 30 nt in...

Descripción completa

Detalles Bibliográficos
Autores principales:	DeStefano, Jeffrey J., Cristofaro, Jason V.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1325207/ https://www.ncbi.nlm.nih.gov/pubmed/16397296 http://dx.doi.org/10.1093/nar/gkj426

Ejemplares similares

Viral Reverse Transcriptases Show Selective High Affinity Binding to DNA-DNA Primer-Templates that Resemble the Polypurine Tract
por: Nair, Gauri R., et al.
Publicado: (2012)

Selection of Primer–Template Sequences That Bind with Enhanced Affinity to Vaccinia Virus E9 DNA Polymerase
por: DeStefano, Jeffrey J., et al.
Publicado: (2022)

Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity
por: Alves Ferreira-Bravo, Irani, et al.
Publicado: (2015)

Novel mutations in Moloney Murine Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer
por: Arezi, Bahram, et al.
Publicado: (2009)

Interactions between HIV-1 Reverse Transcriptase and the Downstream Template Strand in Stable Complexes with Primer-Template
por: Rutvisuttinunt, Wiriya, et al.
Publicado: (2008)

Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer
por: Tuske, Steve, et al.
Publicado: (2020)

Duplex structural differences and not 2′-hydroxyls explain the more stable binding of HIV-reverse transcriptase to RNA-DNA versus DNA-DNA
por: Olimpo, Jeffrey T., et al.
Publicado: (2010)

Alternative divalent cations (Zn(2+), Co(2+), and Mn(2+)) are not mutagenic at conditions optimal for HIV-1 reverse transcriptase activity
por: Achuthan, Vasudevan, et al.
Publicado: (2015)

Mechanism of polypurine tract primer generation by HIV-1 reverse transcriptase
por: Figiel, Małgorzata, et al.
Publicado: (2018)

Direct tracking of reverse-transcriptase speed and template sensitivity: implications for sequencing and analysis of long RNA molecules
por: Guo, Li-Tao, et al.
Publicado: (2022)

HIV Reverse Transcriptase Pre-Steady-State Kinetic Analysis of Chain Terminators and Translocation Inhibitors Reveals Interactions between Magnesium and Nucleotide 3′-OH
por: Dilmore, Christopher R., et al.
Publicado: (2021)

Human telomerase reverse transcriptase binds to a pre-organized hTR in vivo exposing its template
por: Zemora, Georgeta, et al.
Publicado: (2016)

Base Preferences in Non-Templated Nucleotide Incorporation by MMLV-Derived Reverse Transcriptases
por: Zajac, Pawel, et al.
Publicado: (2013)

Downstream Oligonucleotides Strongly Enhance the Affinity of GMP to RNA Primer–Template Complexes
por: Tam, Chun Pong, et al.
Publicado: (2017)

Variation of Human Immunodeficiency Virus Type-1 Reverse Transcriptase within the Simian Immunodeficiency Virus Genome of RT-SHIV
por: Wadford, Debra A., et al.
Publicado: (2014)

Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms
por: Seetaha, Supaphorn, et al.
Publicado: (2020)

Chaperone activation of the hepadnaviral reverse transcriptase for template RNA binding is established by the Hsp70 and stimulated by the Hsp90 system
por: Stahl, Michael, et al.
Publicado: (2007)

Crystal Structure of a Retroviral Polyprotein: Prototype Foamy Virus Protease-Reverse Transcriptase (PR-RT)
por: Harrison, Jerry Joe E. K., et al.
Publicado: (2021)

Human Immunodeficiency Virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells
por: Davis, Adam J, et al.
Publicado: (2008)

Ribonucleoside Triphosphates as Substrate of Human Immunodeficiency Virus Type 1 Reverse Transcriptase in Human Macrophages
por: Kennedy, Edward M., et al.
Publicado: (2010)

Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera
por: Nagumo, Toshiyuki, et al.
Publicado: (1992)

Selective killing of human immunodeficiency virus infected cells by non-nucleoside reverse transcriptase inhibitor-induced activation of HIV protease
por: Jochmans, Dirk, et al.
Publicado: (2010)

Non-templated addition and template switching by Moloney murine leukemia virus (MMLV)-based reverse transcriptases co-occur and compete with each other
por: Wulf, Madalee G., et al.
Publicado: (2019)

HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells
por: Lemay, Julie, et al.
Publicado: (2008)

Differential Binding of Tenofovir and Adefovir to Reverse Transcriptase of Hepatitis B Virus
por: van Hemert, Formijn J., et al.
Publicado: (2014)

Theoretical investigation of the interactions in binding pocket of Reverse Transcriptase
por: Sahu, Kamlesh Kumar, et al.
Publicado: (2015)

NMR characterization of HIV-1 reverse transcriptase binding to various non-nucleoside reverse transcriptase inhibitors with different activities
por: Thammaporn, Ratsupa, et al.
Publicado: (2015)

Phosphoryl guanidine oligonucleotides as primers for RNA-dependent DNA synthesis using murine leukemia virus reverse transcriptase
por: Dyudeeva, E.S., et al.
Publicado: (2022)

Computational drug design strategies applied to the modelling of human immunodeficiency virus-1 reverse transcriptase inhibitors
por: Santos, Lucianna Helene, et al.
Publicado: (2015)

Biochemical and structural comparisons of non-nucleoside reverse transcriptase inhibitors against feline and human immunodeficiency viruses
por: Rattanabunyong, Siriluk, et al.
Publicado: (2023)

2′-fluoro-modified pyrimidines enhance affinity of RNA oligonucleotides to HIV-1 reverse transcriptase
por: Gruenke, Paige R., et al.
Publicado: (2020)

Selection of 2′-Deoxy-2′-Fluoroarabino Nucleic Acid (FANA) Aptamers That Bind HIV-1 Integrase with Picomolar Affinity
por: Rose, Kevin M., et al.
Publicado: (2019)

Trans-lesion synthesis and RNaseH activity by reverse transcriptases on a true abasic RNA template
por: Küpfer, Pascal A., et al.
Publicado: (2007)

Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching
por: Wu, Douglas C., et al.
Publicado: (2017)

Revealing the Mutation Patterns of Drug-Resistant Reverse Transcriptase Variants of Human Immunodeficiency Virus through Proteochemometric Modeling
por: Qiu, Jingxuan, et al.
Publicado: (2021)

Low-bias ncRNA libraries using ordered two-template relay: Serial template jumping by a modified retroelement reverse transcriptase
por: Upton, Heather E., et al.
Publicado: (2021)

Examining the ribonuclease H primer grip of HIV-1 reverse transcriptase by charge neutralization of RNA/DNA hybrids
por: Dash, Chandravanu, et al.
Publicado: (2008)

Structural basis for template switching by a group II intron–encoded non-LTR-retroelement reverse transcriptase
por: Lentzsch, Alfred M., et al.
Publicado: (2021)

Functional Interactions of Kluyveromyces lactis Telomerase Reverse Transcriptase with the Three-Way Junction and the Template Domains of Telomerase RNA
por: Al-Shareef, Wasif, et al.
Publicado: (2022)

APOBEC3 selects V179I in HIV-1 reverse transcriptase to provide selective advantage for non-nucleoside reverse transcriptase inhibitor-resistant mutants
por: Dwivedi, Richa, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_eufks8utvm70erln3jdp9uidkt