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Real-time observation of DNA looping dynamics of Type IIE restriction enzymes NaeI and NarI

Many restriction enzymes require binding of two copies of a recognition sequence for DNA cleavage, thereby introducing a loop in the DNA. We investigated looping dynamics of Type IIE restriction enzymes NaeI and NarI by tracking the Brownian motion of single tethered DNA molecules. DNA containing tw...

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Detalles Bibliográficos
Autores principales: van den Broek, Bram, Vanzi, Francesco, Normanno, Davide, Pavone, Francesco S., Wuite, Gijs J.L.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1326248/
https://www.ncbi.nlm.nih.gov/pubmed/16407332
http://dx.doi.org/10.1093/nar/gkj432