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Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda

BACKGROUND: Alternative means of malaria control are urgently needed. Evaluating the effectiveness of measures that involve genetic manipulation of vector populations will be facilitated by identifying small, genetically isolated vector populations. The study was designed to use variation in microsa...

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Autores principales: Kayondo, Jonathan K, Mukwaya, Louis G, Stump, Aram, Michel, Andrew P, Coulibaly, Mamadou B, Besansky, Nora J, Collins, Frank H
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1327676/
https://www.ncbi.nlm.nih.gov/pubmed/16336684
http://dx.doi.org/10.1186/1475-2875-4-59
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author Kayondo, Jonathan K
Mukwaya, Louis G
Stump, Aram
Michel, Andrew P
Coulibaly, Mamadou B
Besansky, Nora J
Collins, Frank H
author_facet Kayondo, Jonathan K
Mukwaya, Louis G
Stump, Aram
Michel, Andrew P
Coulibaly, Mamadou B
Besansky, Nora J
Collins, Frank H
author_sort Kayondo, Jonathan K
collection PubMed
description BACKGROUND: Alternative means of malaria control are urgently needed. Evaluating the effectiveness of measures that involve genetic manipulation of vector populations will be facilitated by identifying small, genetically isolated vector populations. The study was designed to use variation in microsatellite markers to look at genetic structure across four Lake Victoria islands and two surrounding mainland populations and for evidence of any restriction to free gene flow. METHODS: Four Islands (from 20–50 km apart) and two surrounding mainland populations (96 km apart) were studied. Samples of indoor resting adult mosquitoes, collected over two consecutive years, were genotyped at microsatellite loci distributed broadly throughout the genome and analysed for genetic structure, effective migration (Nem) and effective population size (Ne). RESULTS: Ne estimates showed island populations to consist of smaller demes compared to the mainland ones. Most populations were significantly differentiated geographically, and from one year to the other. Average geographic pair-wise FST ranged from 0.014–0.105 and several pairs of populations had Ne m < 3. The loci showed broad heterogeneity at capturing or estimating population differences. CONCLUSION: These island populations are significantly genetically differentiated. Differences reoccurred over the study period, between the two mainland populations and between each other. This appears to be the product of their separation by water, dynamics of small populations and local adaptation. With further characterisation these islands could become possible sites for applying measures evaluating effectiveness of control by genetic manipulation.
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spelling pubmed-13276762006-01-14 Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda Kayondo, Jonathan K Mukwaya, Louis G Stump, Aram Michel, Andrew P Coulibaly, Mamadou B Besansky, Nora J Collins, Frank H Malar J Research BACKGROUND: Alternative means of malaria control are urgently needed. Evaluating the effectiveness of measures that involve genetic manipulation of vector populations will be facilitated by identifying small, genetically isolated vector populations. The study was designed to use variation in microsatellite markers to look at genetic structure across four Lake Victoria islands and two surrounding mainland populations and for evidence of any restriction to free gene flow. METHODS: Four Islands (from 20–50 km apart) and two surrounding mainland populations (96 km apart) were studied. Samples of indoor resting adult mosquitoes, collected over two consecutive years, were genotyped at microsatellite loci distributed broadly throughout the genome and analysed for genetic structure, effective migration (Nem) and effective population size (Ne). RESULTS: Ne estimates showed island populations to consist of smaller demes compared to the mainland ones. Most populations were significantly differentiated geographically, and from one year to the other. Average geographic pair-wise FST ranged from 0.014–0.105 and several pairs of populations had Ne m < 3. The loci showed broad heterogeneity at capturing or estimating population differences. CONCLUSION: These island populations are significantly genetically differentiated. Differences reoccurred over the study period, between the two mainland populations and between each other. This appears to be the product of their separation by water, dynamics of small populations and local adaptation. With further characterisation these islands could become possible sites for applying measures evaluating effectiveness of control by genetic manipulation. BioMed Central 2005-12-09 /pmc/articles/PMC1327676/ /pubmed/16336684 http://dx.doi.org/10.1186/1475-2875-4-59 Text en Copyright © 2005 Kayondo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kayondo, Jonathan K
Mukwaya, Louis G
Stump, Aram
Michel, Andrew P
Coulibaly, Mamadou B
Besansky, Nora J
Collins, Frank H
Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda
title Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda
title_full Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda
title_fullStr Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda
title_full_unstemmed Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda
title_short Genetic structure of Anopheles gambiae populations on islands in northwestern Lake Victoria, Uganda
title_sort genetic structure of anopheles gambiae populations on islands in northwestern lake victoria, uganda
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1327676/
https://www.ncbi.nlm.nih.gov/pubmed/16336684
http://dx.doi.org/10.1186/1475-2875-4-59
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