Cargando…

A method for accurate detection of genomic microdeletions using real-time quantitative PCR

BACKGROUND: Quantitative Polymerase Chain Reaction (qPCR) is a well-established method for quantifying levels of gene expression, but has not been routinely applied to the detection of constitutional copy number alterations of human genomic DNA. Microdeletions or microduplications of the human genom...

Descripción completa

Detalles Bibliográficos
Autores principales:	Weksberg, Rosanna, Hughes, Simon, Moldovan, Laura, Bassett, Anne S, Chow, Eva WC, Squire, Jeremy A
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2005
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1327677/ https://www.ncbi.nlm.nih.gov/pubmed/16351727 http://dx.doi.org/10.1186/1471-2164-6-180

Ejemplares similares

22q11.2 microdeletion and increased risk for type 2 diabetes
por: Van, Lily, et al.
Publicado: (2020)

Quantitative Real-Time PCR
por: Sluijter, J. P. G., et al.
Publicado: (2006)

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
por: Vandesompele, Jo, et al.
Publicado: (2002)

Digital PCR dynamic range is approaching that of real-time quantitative PCR
por: Jones, Gerwyn M., et al.
Publicado: (2016)

Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR
por: Higginson, Ellen E., et al.
Publicado: (2022)

Simple, Rapid and Inexpensive Quantitative Fluorescent PCR Method for Detection of Microdeletion and Microduplication Syndromes
por: Stofanko, Martin, et al.
Publicado: (2013)

Quantitative real-time PCR analysis of bacterial biomarkers enable fast and accurate monitoring in inflammatory bowel disease
por: Sezgin, Efe, et al.
Publicado: (2022)

A One-Step Real-Time Multiplex PCR for Screening Y-Chromosomal Microdeletions without Downstream Amplicon Size Analysis
por: Kozina, Viviana, et al.
Publicado: (2011)

Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data
por: Artico, Sinara, et al.
Publicado: (2010)

Validation of Reference Genes for Accurate Normalization of Gene Expression in Lilium davidii var. unicolor for Real Time Quantitative PCR
por: Li, XueYan, et al.
Publicado: (2015)

Accurate, Fast and Cost-Effective Diagnostic Test for Monosomy 1p36 Using Real-Time Quantitative PCR
por: Cunha, Pricila da Silva, et al.
Publicado: (2014)

Accurate bulk quantitation of droplet digital PCR
por: Sun, Chen, et al.
Publicado: (2021)

Making standards for quantitative real-time pneumococcal PCR
por: Morpeth, Susan C., et al.
Publicado: (2014)

Clinical Applications of Quantitative Real-Time PCR in Virology
por: Engstrom-Melnyk, Julia, et al.
Publicado: (2015)

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data
por: Boggy, Gregory J., et al.
Publicado: (2010)

Accurate Quantification of AAV Vector Genomes by Quantitative PCR
por: Martinez-Fernandez de la Camara, Cristina, et al.
Publicado: (2021)

Real-time PCR quantitation of glucocorticoid receptor alpha isoform
por: Melo, Murilo R, et al.
Publicado: (2004)

Development of real time PCR for detection and quantitation of Dengue Viruses
por: Gurukumar, KR, et al.
Publicado: (2009)

Detection of viral aerosols by use of real-time quantitative PCR
por: Wen, Zhanbo, et al.
Publicado: (2009)

ANKRD11 pathogenic variants and 16q24.3 microdeletions share an altered DNA methylation signature in patients with KBG syndrome
por: Awamleh, Zain, et al.
Publicado: (2022)

Adult expression of a 3q13.31 microdeletion
por: Lowther, Chelsea, et al.
Publicado: (2014)

Immune-Related Gene Expression Profile in Laboratory Common Marmosets Assessed by an Accurate Quantitative Real-Time PCR Using Selected Reference Genes
por: Fujii, Yoshiki, et al.
Publicado: (2013)

Assessing Reference Genes for Accurate Transcript Normalization Using Quantitative Real-Time PCR in Pearl Millet [Pennisetum glaucum (L.) R. Br.]
por: Saha, Prasenjit, et al.
Publicado: (2014)

Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri
por: Verbrugghe, Phebe, et al.
Publicado: (2021)

HTqPCR: high-throughput analysis and visualization of quantitative real-time PCR data in R
por: Dvinge, Heidi, et al.
Publicado: (2009)

Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis
por: Bujold, Adina R, et al.
Publicado: (2015)

Validation of Reference Genes for Real-Time Quantitative PCR (qPCR) Analysis of Avibacterium paragallinarum
por: Wen, Shuxiang, et al.
Publicado: (2016)

Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)
por: Forootan, Amin, et al.
Publicado: (2017)

Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR
por: Maheshwari, Yogita, et al.
Publicado: (2017)

Comparison of Quantitative Real-Time PCR and Digital PCR to Detect the Polyomavirus in Merkel Cell Carcinoma
por: Barchitta, Martina, et al.
Publicado: (2022)

Comparison of DNA extraction kits and modification of DNA elution procedure for the quantitation of subdominant bacteria from piggery effluents with real-time PCR
por: Desneux, Jérémy, et al.
Publicado: (2014)

Evaluation of Reference Genes and Normalization Strategy for Quantitative Real-Time PCR in Human Pancreatic Carcinoma
por: Mohelnikova-Duchonova, Beatrice, et al.
Publicado: (2012)

Examination of Y-Chromosomal Microdeletions and Partial Microdeletions in Idiopathic Infertility in East Hungarian Patients
por: Mokánszki, Attila, et al.
Publicado: (2018)

Quantitative Detection of Schistosoma japonicum Cercariae in Water by Real-Time PCR
por: Hung, Yuen Wai, et al.
Publicado: (2008)

Quantitative biomarker analysis of synovial gene expression by real-time PCR
por: Boyle, David L, et al.
Publicado: (2003)

Comparison of Real-Time Quantitative PCR and Culture for the Diagnosis of Emerging Rickettsioses
por: Angelakis, Emmanouil, et al.
Publicado: (2012)

Estimating the number of integrations in transformed plants by quantitative real-time PCR
por: Mason, Giovanna, et al.
Publicado: (2002)

Conventional versus real‐time quantitative PCR for rare species detection
por: Xia, Zhiqiang, et al.
Publicado: (2018)

Quantitative real-time PCR for the measurement of feline cytokine mRNA
por: Leutenegger, Christian M, et al.
Publicado: (1999)

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry
por: Spiess, Andrej-Nikolai, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_fp8q6vdckp9pqli83ej0orjbr4