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Rapid and simple comparison of messenger RNA levels using real-time PCR
Real-time polymerase chain reaction (PCR) constitutes a significant improvement over traditional end-point PCR, as it allows the quantification of starting amounts of nucleic acid templates, in real-time. However, quantification requires validation through numerous internal controls and standard cur...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Biological Procedures Online
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1352391/ https://www.ncbi.nlm.nih.gov/pubmed/16446781 http://dx.doi.org/10.1251/bpo114 |