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Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray

Cytoplasmic control of the adenylation state of mRNAs is a critical post-transcriptional process involved in the regulation of mRNAs stability and translational efficiency. The early development of Xenopus laevis has been a major model for the study of such regulations. We describe here a microarray...

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Detalles Bibliográficos
Autores principales: Graindorge, Antoine, Thuret, Raphaël, Pollet, Nicolas, Osborne, H. Beverley, Audic, Yann
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1361620/
https://www.ncbi.nlm.nih.gov/pubmed/16464828
http://dx.doi.org/10.1093/nar/gkj492
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author Graindorge, Antoine
Thuret, Raphaël
Pollet, Nicolas
Osborne, H. Beverley
Audic, Yann
author_facet Graindorge, Antoine
Thuret, Raphaël
Pollet, Nicolas
Osborne, H. Beverley
Audic, Yann
author_sort Graindorge, Antoine
collection PubMed
description Cytoplasmic control of the adenylation state of mRNAs is a critical post-transcriptional process involved in the regulation of mRNAs stability and translational efficiency. The early development of Xenopus laevis has been a major model for the study of such regulations. We describe here a microarray analysis to identify mRNAs that are regulated by changes in their adenylation state during oogenesis and early development of the diploid frog Xenopus tropicalis. The microarray data were validated using qRT–PCR and direct analysis of the adenylation state of endogenous maternal mRNAs during the period studied. We identified more than 500 mRNAs regulated at the post-transcriptional level among the 3000 mRNAs potentially detected by the microarray. The mRNAs were classified into nine different adenylation behavior categories. The various adenylation profiles observed during oocyte maturation and early development and the analyses of 3′-untranslated region sequences suggest that previously uncharacterized sequence elements control the adenylation behavior of the newly identified mRNAs. These data should prove useful in identifying mRNAs with important functions during oocyte maturation and early development.
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spelling pubmed-13616202006-02-09 Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray Graindorge, Antoine Thuret, Raphaël Pollet, Nicolas Osborne, H. Beverley Audic, Yann Nucleic Acids Res Article Cytoplasmic control of the adenylation state of mRNAs is a critical post-transcriptional process involved in the regulation of mRNAs stability and translational efficiency. The early development of Xenopus laevis has been a major model for the study of such regulations. We describe here a microarray analysis to identify mRNAs that are regulated by changes in their adenylation state during oogenesis and early development of the diploid frog Xenopus tropicalis. The microarray data were validated using qRT–PCR and direct analysis of the adenylation state of endogenous maternal mRNAs during the period studied. We identified more than 500 mRNAs regulated at the post-transcriptional level among the 3000 mRNAs potentially detected by the microarray. The mRNAs were classified into nine different adenylation behavior categories. The various adenylation profiles observed during oocyte maturation and early development and the analyses of 3′-untranslated region sequences suggest that previously uncharacterized sequence elements control the adenylation behavior of the newly identified mRNAs. These data should prove useful in identifying mRNAs with important functions during oocyte maturation and early development. Oxford University Press 2006 2006-02-07 /pmc/articles/PMC1361620/ /pubmed/16464828 http://dx.doi.org/10.1093/nar/gkj492 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Article
Graindorge, Antoine
Thuret, Raphaël
Pollet, Nicolas
Osborne, H. Beverley
Audic, Yann
Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray
title Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray
title_full Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray
title_fullStr Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray
title_full_unstemmed Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray
title_short Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray
title_sort identification of post-transcriptionally regulated xenopus tropicalis maternal mrnas by microarray
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1361620/
https://www.ncbi.nlm.nih.gov/pubmed/16464828
http://dx.doi.org/10.1093/nar/gkj492
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