Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage φC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the co...

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Detalles Bibliográficos
Autores principales: Khodakaramian, Gholam, Lissenden, Sarah, Gust, Bertolt, Moir, Laura, Hoskisson, Paul A., Chater, Keith F., Smith, Margaret C. M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363781/
https://www.ncbi.nlm.nih.gov/pubmed/16473843
http://dx.doi.org/10.1093/nar/gnj019
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author Khodakaramian, Gholam
Lissenden, Sarah
Gust, Bertolt
Moir, Laura
Hoskisson, Paul A.
Chater, Keith F.
Smith, Margaret C. M.
author_facet Khodakaramian, Gholam
Lissenden, Sarah
Gust, Bertolt
Moir, Laura
Hoskisson, Paul A.
Chater, Keith F.
Smith, Margaret C. M.
author_sort Khodakaramian, Gholam
collection PubMed
description We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage φC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
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spelling pubmed-13637812006-02-14 Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces Khodakaramian, Gholam Lissenden, Sarah Gust, Bertolt Moir, Laura Hoskisson, Paul A. Chater, Keith F. Smith, Margaret C. M. Nucleic Acids Res Methods Online We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage φC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor. Oxford University Press 2006 2006-02-09 /pmc/articles/PMC1363781/ /pubmed/16473843 http://dx.doi.org/10.1093/nar/gnj019 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Methods Online
Khodakaramian, Gholam
Lissenden, Sarah
Gust, Bertolt
Moir, Laura
Hoskisson, Paul A.
Chater, Keith F.
Smith, Margaret C. M.
Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
title Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
title_full Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
title_fullStr Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
title_full_unstemmed Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
title_short Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
title_sort expression of cre recombinase during transient phage infection permits efficient marker removal in streptomyces
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363781/
https://www.ncbi.nlm.nih.gov/pubmed/16473843
http://dx.doi.org/10.1093/nar/gnj019
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