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Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage φC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the co...

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Detalles Bibliográficos
Autores principales:	Khodakaramian, Gholam, Lissenden, Sarah, Gust, Bertolt, Moir, Laura, Hoskisson, Paul A., Chater, Keith F., Smith, Margaret C. M.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363781/ https://www.ncbi.nlm.nih.gov/pubmed/16473843 http://dx.doi.org/10.1093/nar/gnj019

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