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Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis

BACKGROUND: T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinom...

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Detalles Bibliográficos
Autores principales:	Zhao, Hongjuan, Hastie, Trevor, Whitfield, Michael L, Børresen-Dale, Anne-Lise, Jeffrey, Stefanie S
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2002
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC137577/ https://www.ncbi.nlm.nih.gov/pubmed/12445333 http://dx.doi.org/10.1186/1471-2164-3-31

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