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The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation
Affinity tagging has been used in many global studies towards protein function. We describe a highly efficient system for in vivo biotinylation of transcription factors in the yeast Saccharomyces cerevisiae, which is based on the bacterial BirA biotin ligase. The strength of the biotin–streptavidin...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1383622/ https://www.ncbi.nlm.nih.gov/pubmed/16500888 http://dx.doi.org/10.1093/nar/gkl003 |