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Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons

BACKGROUND: Targeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity. In order to gain an overview of the composition of the dendritic mRNA pool, we dissected out stratum radiatum (dendritic lamina) from rat hippocampal CA1 region and com...

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Detalles Bibliográficos
Autores principales: Zhong, Jun, Zhang, Theresa, Bloch, Lisa M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1386695/
https://www.ncbi.nlm.nih.gov/pubmed/16503994
http://dx.doi.org/10.1186/1471-2202-7-17
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author Zhong, Jun
Zhang, Theresa
Bloch, Lisa M
author_facet Zhong, Jun
Zhang, Theresa
Bloch, Lisa M
author_sort Zhong, Jun
collection PubMed
description BACKGROUND: Targeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity. In order to gain an overview of the composition of the dendritic mRNA pool, we dissected out stratum radiatum (dendritic lamina) from rat hippocampal CA1 region and compared its mRNA content with that of stratum pyramidale (cell body layer) using a set of cDNA microarrays. RNAs that have over-representation in the dendritic fraction were annotated and sorted into function groups. RESULTS: We have identified 154 dendritic mRNA candidates, which can be arranged into the categories of receptors and channels, signaling molecules, cytoskeleton and adhesion molecules, and factors that are involved in membrane trafficking, in protein synthesis, in posttranslational protein modification, and in protein degradation. Previously known dendritic mRNAs such as MAP2, calmodulin, and G protein gamma subunit were identified from our screening, as were mRNAs that encode proteins known to be important for synaptic plasticity and memory, such as spinophilin, Pumilio, eEF1A, and MHC class I molecules. Furthermore, mRNAs coding for ribosomal proteins were also found in dendrites. CONCLUSION: Our results suggest that neurons transport a variety of mRNAs to dendrites, not only those directly involved in modulating synaptic plasticity, but also others that play more common roles in cellular metabolism.
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spelling pubmed-13866952006-03-02 Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons Zhong, Jun Zhang, Theresa Bloch, Lisa M BMC Neurosci Research Article BACKGROUND: Targeted transport of messenger RNA and local protein synthesis near the synapse are important for synaptic plasticity. In order to gain an overview of the composition of the dendritic mRNA pool, we dissected out stratum radiatum (dendritic lamina) from rat hippocampal CA1 region and compared its mRNA content with that of stratum pyramidale (cell body layer) using a set of cDNA microarrays. RNAs that have over-representation in the dendritic fraction were annotated and sorted into function groups. RESULTS: We have identified 154 dendritic mRNA candidates, which can be arranged into the categories of receptors and channels, signaling molecules, cytoskeleton and adhesion molecules, and factors that are involved in membrane trafficking, in protein synthesis, in posttranslational protein modification, and in protein degradation. Previously known dendritic mRNAs such as MAP2, calmodulin, and G protein gamma subunit were identified from our screening, as were mRNAs that encode proteins known to be important for synaptic plasticity and memory, such as spinophilin, Pumilio, eEF1A, and MHC class I molecules. Furthermore, mRNAs coding for ribosomal proteins were also found in dendrites. CONCLUSION: Our results suggest that neurons transport a variety of mRNAs to dendrites, not only those directly involved in modulating synaptic plasticity, but also others that play more common roles in cellular metabolism. BioMed Central 2006-02-17 /pmc/articles/PMC1386695/ /pubmed/16503994 http://dx.doi.org/10.1186/1471-2202-7-17 Text en Copyright © 2006 Zhong et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhong, Jun
Zhang, Theresa
Bloch, Lisa M
Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons
title Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons
title_full Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons
title_fullStr Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons
title_full_unstemmed Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons
title_short Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neurons
title_sort dendritic mrnas encode diversified functionalities in hippocampal pyramidal neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1386695/
https://www.ncbi.nlm.nih.gov/pubmed/16503994
http://dx.doi.org/10.1186/1471-2202-7-17
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