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HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain
BACKGROUND: To examine the role of inflammatory mediators in neuropathic pain, we used a replication-defective genomic herpes simplex virus (HSV)-based vector containing the coding sequence for the anti-inflammatory peptide interleukin (IL)-4 under the transcriptional control of the HSV ICP4 immedia...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1395302/ https://www.ncbi.nlm.nih.gov/pubmed/16503976 http://dx.doi.org/10.1186/1744-8069-2-6 |
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author | Hao, Shuanglin Mata, Marina Glorioso, Joseph C Fink, David J |
author_facet | Hao, Shuanglin Mata, Marina Glorioso, Joseph C Fink, David J |
author_sort | Hao, Shuanglin |
collection | PubMed |
description | BACKGROUND: To examine the role of inflammatory mediators in neuropathic pain, we used a replication-defective genomic herpes simplex virus (HSV)-based vector containing the coding sequence for the anti-inflammatory peptide interleukin (IL)-4 under the transcriptional control of the HSV ICP4 immediate early promoter, vector S4IL4, to express IL-4 in dorsal root ganglion (DRG) neurons in vivo. RESULTS: Subcutaneous inoculation of S4IL4 in the foot transduced lumbar DRG to produce IL-4. Transgene-mediated expression of IL-4 did not alter thermal latency or tactile threshold in normal animals, but inoculation of S4IL4 1 week after spinal nerve ligation (SNL) reduced mechanical allodynia and reversed thermal hyperalgesia resulting from SNL. Inoculation of S4IL4 1 week before SNL delayed the development of thermal hyperalgesia and tactile allodynia, but did not prevent the ultimate development of these manifestations of neuropathic pain. S4IL4 inoculation suppressed non-noxious-induced expression of c-Fos immunoreactivity in dorsal horn of spinal cord and reversed the upregulation of spinal IL-1β, PGE2, and phosphorylated-p38 MAP kinase, characteristic of neuropathic pain. CONCLUSION: HSV-mediated expression of IL-4 effectively reduces the behavioral manifestations of neuropathic pain, and reverses some of the biochemical and histologic correlates of neuropathic pain at the spinal level. |
format | Text |
id | pubmed-1395302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-13953022006-03-09 HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain Hao, Shuanglin Mata, Marina Glorioso, Joseph C Fink, David J Mol Pain Research BACKGROUND: To examine the role of inflammatory mediators in neuropathic pain, we used a replication-defective genomic herpes simplex virus (HSV)-based vector containing the coding sequence for the anti-inflammatory peptide interleukin (IL)-4 under the transcriptional control of the HSV ICP4 immediate early promoter, vector S4IL4, to express IL-4 in dorsal root ganglion (DRG) neurons in vivo. RESULTS: Subcutaneous inoculation of S4IL4 in the foot transduced lumbar DRG to produce IL-4. Transgene-mediated expression of IL-4 did not alter thermal latency or tactile threshold in normal animals, but inoculation of S4IL4 1 week after spinal nerve ligation (SNL) reduced mechanical allodynia and reversed thermal hyperalgesia resulting from SNL. Inoculation of S4IL4 1 week before SNL delayed the development of thermal hyperalgesia and tactile allodynia, but did not prevent the ultimate development of these manifestations of neuropathic pain. S4IL4 inoculation suppressed non-noxious-induced expression of c-Fos immunoreactivity in dorsal horn of spinal cord and reversed the upregulation of spinal IL-1β, PGE2, and phosphorylated-p38 MAP kinase, characteristic of neuropathic pain. CONCLUSION: HSV-mediated expression of IL-4 effectively reduces the behavioral manifestations of neuropathic pain, and reverses some of the biochemical and histologic correlates of neuropathic pain at the spinal level. BioMed Central 2006-02-17 /pmc/articles/PMC1395302/ /pubmed/16503976 http://dx.doi.org/10.1186/1744-8069-2-6 Text en Copyright © 2006 Hao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Hao, Shuanglin Mata, Marina Glorioso, Joseph C Fink, David J HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain |
title | HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain |
title_full | HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain |
title_fullStr | HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain |
title_full_unstemmed | HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain |
title_short | HSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain |
title_sort | hsv-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1395302/ https://www.ncbi.nlm.nih.gov/pubmed/16503976 http://dx.doi.org/10.1186/1744-8069-2-6 |
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