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Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa

BACKGROUND: To apply clarithromycin as an immunomodulatory treatment in experimental urosepsis by multidrug-resistant Pseudomonas aeruginosa. METHODS: Acute pyelonephritis was induced in 40 rabbits after inoculation of the test isolate in the renal pelvis. Therapy was administered upon signs of seps...

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Autores principales: Giamarellos-Bourboulis, Evangelos J, Antonopoulou, Anastasia, Raftogiannis, Maria, Koutoukas, Pantelis, Tsaganos, Thomas, Tziortzioti, Vassiliki, Panagou, Charalambos, Adamis, Theodoros, Giamarellou, Helen
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1397846/
https://www.ncbi.nlm.nih.gov/pubmed/16504031
http://dx.doi.org/10.1186/1471-2334-6-31
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author Giamarellos-Bourboulis, Evangelos J
Antonopoulou, Anastasia
Raftogiannis, Maria
Koutoukas, Pantelis
Tsaganos, Thomas
Tziortzioti, Vassiliki
Panagou, Charalambos
Adamis, Theodoros
Giamarellou, Helen
author_facet Giamarellos-Bourboulis, Evangelos J
Antonopoulou, Anastasia
Raftogiannis, Maria
Koutoukas, Pantelis
Tsaganos, Thomas
Tziortzioti, Vassiliki
Panagou, Charalambos
Adamis, Theodoros
Giamarellou, Helen
author_sort Giamarellos-Bourboulis, Evangelos J
collection PubMed
description BACKGROUND: To apply clarithromycin as an immunomodulatory treatment in experimental urosepsis by multidrug-resistant Pseudomonas aeruginosa. METHODS: Acute pyelonephritis was induced in 40 rabbits after inoculation of the test isolate in the renal pelvis. Therapy was administered upon signs of sepsis in four groups: A, controls; B, intravenous clarithromycin; C, amikacin; and D, both agents. Survival and vital signs were recorded; blood was sampled for culture and estimation of pro-inflammatory mediators; monocytes were isolated for determination of apoptotic rate and ex vivo TNFα secretion. Quantitative cultures and biopsies of organs were performed after death. RESULTS: Increased rectal temperature and oxygen saturation were found in groups B and D compared to A and C. Mean survival of groups A, B, C and D was 2.65, 7.15, 4.25 and 8.70 days respectively. No differences were noted between groups concerning bacterial load in blood and tissues and serum endotoxins. Serum MDA and total caspase-3 activity of monocytes of group D decreased following treatment compared to other groups. Negative correlation was detected between cytoplasmic caspase-3 and ex vivo secretion of TNFα of blood monocytes of group A; similar correlation was not found for any other group. Pathology scores of liver and lung of group B were lower than group A. CONCLUSION: Clarithromycin administered late in experimental urosepsis by multidrug-resistant P. aeruginosa prolonged survival and ameliorated clinical findings. Its effect is probably attributed to immunomodulatory intervention on blood monocytes.
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spelling pubmed-13978462006-03-11 Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa Giamarellos-Bourboulis, Evangelos J Antonopoulou, Anastasia Raftogiannis, Maria Koutoukas, Pantelis Tsaganos, Thomas Tziortzioti, Vassiliki Panagou, Charalambos Adamis, Theodoros Giamarellou, Helen BMC Infect Dis Research Article BACKGROUND: To apply clarithromycin as an immunomodulatory treatment in experimental urosepsis by multidrug-resistant Pseudomonas aeruginosa. METHODS: Acute pyelonephritis was induced in 40 rabbits after inoculation of the test isolate in the renal pelvis. Therapy was administered upon signs of sepsis in four groups: A, controls; B, intravenous clarithromycin; C, amikacin; and D, both agents. Survival and vital signs were recorded; blood was sampled for culture and estimation of pro-inflammatory mediators; monocytes were isolated for determination of apoptotic rate and ex vivo TNFα secretion. Quantitative cultures and biopsies of organs were performed after death. RESULTS: Increased rectal temperature and oxygen saturation were found in groups B and D compared to A and C. Mean survival of groups A, B, C and D was 2.65, 7.15, 4.25 and 8.70 days respectively. No differences were noted between groups concerning bacterial load in blood and tissues and serum endotoxins. Serum MDA and total caspase-3 activity of monocytes of group D decreased following treatment compared to other groups. Negative correlation was detected between cytoplasmic caspase-3 and ex vivo secretion of TNFα of blood monocytes of group A; similar correlation was not found for any other group. Pathology scores of liver and lung of group B were lower than group A. CONCLUSION: Clarithromycin administered late in experimental urosepsis by multidrug-resistant P. aeruginosa prolonged survival and ameliorated clinical findings. Its effect is probably attributed to immunomodulatory intervention on blood monocytes. BioMed Central 2006-02-21 /pmc/articles/PMC1397846/ /pubmed/16504031 http://dx.doi.org/10.1186/1471-2334-6-31 Text en Copyright © 2006 Giamarellos-Bourboulis et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Giamarellos-Bourboulis, Evangelos J
Antonopoulou, Anastasia
Raftogiannis, Maria
Koutoukas, Pantelis
Tsaganos, Thomas
Tziortzioti, Vassiliki
Panagou, Charalambos
Adamis, Theodoros
Giamarellou, Helen
Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa
title Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa
title_full Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa
title_fullStr Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa
title_full_unstemmed Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa
title_short Clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant Pseudomonas aeruginosa
title_sort clarithromycin is an effective immunomodulator when administered late in experimental pyelonephritis by multidrug-resistant pseudomonas aeruginosa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1397846/
https://www.ncbi.nlm.nih.gov/pubmed/16504031
http://dx.doi.org/10.1186/1471-2334-6-31
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