Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate

Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosome...

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Detalles Bibliográficos
Autores principales: Battista, Natalia, Bari, Monica, Finazzi-Agrò, Alessandro, Maccarrone, Mauro
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Short Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC139962/
https://www.ncbi.nlm.nih.gov/pubmed/12617751
http://dx.doi.org/10.1186/1476-511X-1-1
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author Battista, Natalia
Bari, Monica
Finazzi-Agrò, Alessandro
Maccarrone, Mauro
author_facet Battista, Natalia
Bari, Monica
Finazzi-Agrò, Alessandro
Maccarrone, Mauro
author_sort Battista, Natalia
collection PubMed
description Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.
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spelling pubmed-1399622003-01-20 Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate Battista, Natalia Bari, Monica Finazzi-Agrò, Alessandro Maccarrone, Mauro Lipids Health Dis Short Paper Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT. BioMed Central 2002-09-03 /pmc/articles/PMC139962/ /pubmed/12617751 http://dx.doi.org/10.1186/1476-511X-1-1 Text en Copyright © 2002 Battista et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Short Paper
Battista, Natalia
Bari, Monica
Finazzi-Agrò, Alessandro
Maccarrone, Mauro
Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_full Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_fullStr Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_full_unstemmed Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_short Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_sort anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
topic Short Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC139962/
https://www.ncbi.nlm.nih.gov/pubmed/12617751
http://dx.doi.org/10.1186/1476-511X-1-1
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