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DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria
BACKGROUND: Complete sequencing and annotation of the 96.2 kb Bacillus anthracis plasmid, pXO2, predicted 85 open reading frames (ORFs). Bacillus cereus and Bacillus thuringiensis isolates that ranged in genomic similarity to B. anthracis, as determined by amplified fragment length polymorphism (AFL...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140023/ https://www.ncbi.nlm.nih.gov/pubmed/12473162 http://dx.doi.org/10.1186/1471-2164-3-34 |
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author | Pannucci, James Okinaka, Richard T Williams, Erin Sabin, Robert Ticknor, Lawrence O Kuske, Cheryl R |
author_facet | Pannucci, James Okinaka, Richard T Williams, Erin Sabin, Robert Ticknor, Lawrence O Kuske, Cheryl R |
author_sort | Pannucci, James |
collection | PubMed |
description | BACKGROUND: Complete sequencing and annotation of the 96.2 kb Bacillus anthracis plasmid, pXO2, predicted 85 open reading frames (ORFs). Bacillus cereus and Bacillus thuringiensis isolates that ranged in genomic similarity to B. anthracis, as determined by amplified fragment length polymorphism (AFLP) analysis, were examined by PCR for the presence of sequences similar to 47 pXO2 ORFs. RESULTS: The two most distantly related isolates examined, B. thuringiensis 33679 and B. thuringiensis AWO6, produced the greatest number of ORF sequences similar to pXO2; 10 detected in 33679 and 16 in AWO6. No more than two of the pXO2 ORFs were detected in any one of the remaining isolates. Dot-blot DNA hybridizations between pXO2 ORF fragments and total genomic DNA from AWO6 were consistent with the PCR assay results for this isolate and also revealed nine additional ORFs shared between these two bacteria. Sequences similar to the B. anthracis cap genes or their regulator, acpA, were not detected among any of the examined isolates. CONCLUSIONS: The presence of pXO2 sequences in the other Bacillus isolates did not correlate with genomic relatedness established by AFLP analysis. The presence of pXO2 ORF sequences in other Bacillus species suggests the possibility that certain pXO2 plasmid gene functions may also be present in other closely related bacteria. |
format | Text |
id | pubmed-140023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-1400232003-01-20 DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria Pannucci, James Okinaka, Richard T Williams, Erin Sabin, Robert Ticknor, Lawrence O Kuske, Cheryl R BMC Genomics Research Article BACKGROUND: Complete sequencing and annotation of the 96.2 kb Bacillus anthracis plasmid, pXO2, predicted 85 open reading frames (ORFs). Bacillus cereus and Bacillus thuringiensis isolates that ranged in genomic similarity to B. anthracis, as determined by amplified fragment length polymorphism (AFLP) analysis, were examined by PCR for the presence of sequences similar to 47 pXO2 ORFs. RESULTS: The two most distantly related isolates examined, B. thuringiensis 33679 and B. thuringiensis AWO6, produced the greatest number of ORF sequences similar to pXO2; 10 detected in 33679 and 16 in AWO6. No more than two of the pXO2 ORFs were detected in any one of the remaining isolates. Dot-blot DNA hybridizations between pXO2 ORF fragments and total genomic DNA from AWO6 were consistent with the PCR assay results for this isolate and also revealed nine additional ORFs shared between these two bacteria. Sequences similar to the B. anthracis cap genes or their regulator, acpA, were not detected among any of the examined isolates. CONCLUSIONS: The presence of pXO2 sequences in the other Bacillus isolates did not correlate with genomic relatedness established by AFLP analysis. The presence of pXO2 ORF sequences in other Bacillus species suggests the possibility that certain pXO2 plasmid gene functions may also be present in other closely related bacteria. BioMed Central 2002-12-09 /pmc/articles/PMC140023/ /pubmed/12473162 http://dx.doi.org/10.1186/1471-2164-3-34 Text en Copyright © 2002 Pannucci et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Pannucci, James Okinaka, Richard T Williams, Erin Sabin, Robert Ticknor, Lawrence O Kuske, Cheryl R DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria |
title | DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria |
title_full | DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria |
title_fullStr | DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria |
title_full_unstemmed | DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria |
title_short | DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria |
title_sort | dna sequence conservation between the bacillus anthracis pxo2 plasmid and genomic sequence from closely related bacteria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140023/ https://www.ncbi.nlm.nih.gov/pubmed/12473162 http://dx.doi.org/10.1186/1471-2164-3-34 |
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