Cargando…
Synthesis by High-Efficiency Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight Polyethylene Glycols (PEGs)
The chemical modification of synthetic oligonucleotides has recently been investigated to improve their pharmacological utilization. In addition to chemical alterations of the backbone and of the heterocyclic bases, their conjugation with amphiphylic moieties, such as the polyethylene glycol has bee...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Biological Procedures Online
1998
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140118/ https://www.ncbi.nlm.nih.gov/pubmed/12734594 http://dx.doi.org/10.1251/bpo2 |
_version_ | 1782120596461584384 |
---|---|
author | Bonora, GM Zaramella, S Veronese, FM |
author_facet | Bonora, GM Zaramella, S Veronese, FM |
author_sort | Bonora, GM |
collection | PubMed |
description | The chemical modification of synthetic oligonucleotides has recently been investigated to improve their pharmacological utilization. In addition to chemical alterations of the backbone and of the heterocyclic bases, their conjugation with amphiphylic moieties, such as the polyethylene glycol has been proposed. The large scale production of these molecules as demanded for commercial purposes is hampered by the heterogeneity of the solid-phase processes and by the low reactivity of high-molecular weight PEGs in solution. A new synthetic procedure based on the recently developed liquid-phase method (HELP), has been set up to overcome these limitations. |
format | Text |
id | pubmed-140118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | Biological Procedures Online |
record_format | MEDLINE/PubMed |
spelling | pubmed-1401182006-04-06 Synthesis by High-Efficiency Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight Polyethylene Glycols (PEGs) Bonora, GM Zaramella, S Veronese, FM Biol Proced Online Research Article The chemical modification of synthetic oligonucleotides has recently been investigated to improve their pharmacological utilization. In addition to chemical alterations of the backbone and of the heterocyclic bases, their conjugation with amphiphylic moieties, such as the polyethylene glycol has been proposed. The large scale production of these molecules as demanded for commercial purposes is hampered by the heterogeneity of the solid-phase processes and by the low reactivity of high-molecular weight PEGs in solution. A new synthetic procedure based on the recently developed liquid-phase method (HELP), has been set up to overcome these limitations. Biological Procedures Online 1998-05-14 /pmc/articles/PMC140118/ /pubmed/12734594 http://dx.doi.org/10.1251/bpo2 Text en Copyright © May 05, 1998, GM Bonora et al. Published in Biological Procedures Online under license from the authors. Copying, printing, redistribution and storage permitted. |
spellingShingle | Research Article Bonora, GM Zaramella, S Veronese, FM Synthesis by High-Efficiency Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight Polyethylene Glycols (PEGs) |
title | Synthesis by High-Efficiency
Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight
Polyethylene Glycols (PEGs) |
title_full | Synthesis by High-Efficiency
Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight
Polyethylene Glycols (PEGs) |
title_fullStr | Synthesis by High-Efficiency
Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight
Polyethylene Glycols (PEGs) |
title_full_unstemmed | Synthesis by High-Efficiency
Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight
Polyethylene Glycols (PEGs) |
title_short | Synthesis by High-Efficiency
Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight
Polyethylene Glycols (PEGs) |
title_sort | synthesis by high-efficiency
liquid-phase (help) method of oligonucleotides conjugated with high-molecular weight
polyethylene glycols (pegs) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140118/ https://www.ncbi.nlm.nih.gov/pubmed/12734594 http://dx.doi.org/10.1251/bpo2 |
work_keys_str_mv | AT bonoragm synthesisbyhighefficiencyliquidphasehelpmethodofoligonucleotidesconjugatedwithhighmolecularweightpolyethyleneglycolspegs AT zaramellas synthesisbyhighefficiencyliquidphasehelpmethodofoligonucleotidesconjugatedwithhighmolecularweightpolyethyleneglycolspegs AT veronesefm synthesisbyhighefficiencyliquidphasehelpmethodofoligonucleotidesconjugatedwithhighmolecularweightpolyethyleneglycolspegs |