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Expression of a prokaryotic P-type ATPase in E. coli Plasma Membranes and Purification by Ni(2+)-affinity chromatography
In order to characterize the P-type ATPase from Synechocystis 6803 [Geisler (1993) et al. J. Mol. Biol. 234, 1284] and to facilitate its purification, we expressed an N-terminal 6xHis-tagged version of the ATPase in an ATPase deficient E. coli strain. The expressed ATPase was immunodetected as a dom...
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| Formato: | Texto |
| Lenguaje: | English |
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Biological Procedures Online
1998
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140125/ https://www.ncbi.nlm.nih.gov/pubmed/12734587 http://dx.doi.org/10.1251/bpo9 |