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Single-step purification of full-length human androgen receptor

The full-length human androgen receptor with an N-terminal biotin acceptor peptide tag was overexpressed in Spodoptera frugiperda cells in the presence of 1 µM dihydrotestosterone. Site-specific biotinylation of BAP was achieved in vivo by co-expression of E. coli biotin holoenzyme synthetase. The a...

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Detalles Bibliográficos
Autores principales:	Juzumiene, Dalia, Chang, Ching-yi, Fan, Daju, Hartney, Tanya, Norris, John D., McDonnell, Donald P.
Formato:	Texto
Lenguaje:	English
Publicado:	The Nuclear Receptor Signaling Atlas 2005
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1402216/ https://www.ncbi.nlm.nih.gov/pubmed/16604169 http://dx.doi.org/10.1621/nrs.03001

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